鬼针草总黄酮对肝纤维化大鼠细胞因子的影响

目的观察鬼针草总黄酮(totalflavones of Bidenspilo-saL,TFB)对肝纤维化大鼠细胞因子的影响。方法将大鼠随机分成正常组、对照组、TFB160、80、40mg·kg-1组和秋水仙碱(Col)0.1mg·kg-1阳性药对照组。除正常组外,其余各组采用四氯化碳(CCl4)诱导肝纤维化模型。于造模wk9起,给药组分别灌胃相应的受试药物,正常组和对照组灌胃等容量的生理盐水,疗程10wk。实验结束后,股动脉采血,放免法测定血清中肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)的含量,同时取固定部位肝脏组织,免疫组化技术测定TFB对肝组织中TGF-β1和NF-κB蛋白表达的影响,RT-PCR技术测定TFB对肝组织中TGF-β1 mRNA表达的影响。结果TFB160、80mg·kg-1能明显降低肝纤维化大鼠血清TNF-α、IL-1β含量,抑制肝纤维化大鼠肝组织中NF-κB、TGF-β1蛋白和TGF-β1 mRNA表达。结论TFB通过降低肝纤维化大鼠炎症细胞因子而抗肝纤维化。

Total flavones of Bidens pilosa L's effect on cytokines in liver fibrosis rats

Objectives To study the effect of total flavones of Bidens pilosa L (TFB) on cytokines production in liver fibrosis rats. Methods Rat liver fibrosis was induced by CCl450%, 0.1 ml·(100 g)-1 body weight twice a week for 18 weeks. TFB (160,80,40 mg·kg-1) was used daily via gastrogavage at 9 week. Levels of TNF-α and IL-1β in serum were determinate by radioimmunoassay. Liver samples were collected after experiments and stained by immuninochemistry of TGF-β1 and NF-κB. Moreover TGF-β1 mRNA expression in liver tissue was detected by RT-PCR technology. Results TFB (160, 80 mg·kg-1) could significantly reduce serum TNF-α and IL-1β contents; TFB(160, 80, 40 mg·kg-1) could effectively prevent the expression of NF-κB,as was TGF-β1 of TFB(160, 80 mg·kg-1). Moreover TFB (160, 80 mg·kg-1) could significantly reduce TGF-β1 mRNA in liver fibrosis rats. Conclusion TFB had protective effect on liver fibrosis by its inhibition of cytokine production.