慢性应激大鼠抑郁模型的建立及其有效性的探讨

目的探讨建立慢性应激抑郁大鼠模型的实验方法及此模型的有效性。方法利用慢性不可预见的中等强度应激制作抑郁模型，观察动物的体重和摄食量的变化，用敞箱试验及液体消耗实验测定动物行为。结果大鼠抑郁模型在第28天建立成功。抑郁组大鼠体重增加量、摄食量、敞箱试验得分、液体消耗试验中糖水消耗和糖水偏爱百分比均明显下降，纯水消耗显著提高。结论慢性不可预见的中等强度应激可以成功建立大鼠抑郁模型。

Establishment and Evaluation of Rat Model of Chronic Unpredictable Stress Depression

Objective To explore the validity and reliability of experimental rat model of chronic unpredictable depression.Methods The rat model of depression was established by exposing to an unpredictable sequence of stressors. The changes in body weight and food intake in the animals were observed. By openfield test and fluid consumption test, the behaviors of the animals were determined.Results The rat model of depression has been established successfully at 28th day. In the depression group, the rats showed decreased body weight and food intake, reduced squares crossing and rearing in openfield test, a significantly reduced consumption of and preference for sucrose solutions and an increased pure water consumption as compared with control group.Conclusion The rat model of depression can be established successfully by exposing to an unpredictable sequence of stressors.