| 黄曲霉毒素B1完全抗原构建中结合位点研究 |
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| 引用本文： | 孙秀兰,张银志,汤坚,朱瑜.黄曲霉毒素B1完全抗原构建中结合位点研究[J].中国脊柱脊髓杂志,2007(5). |
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| 作者姓名： | 孙秀兰 张银志 汤坚 朱瑜 |
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| 作者单位： | [1]江南大学食品学院,江苏无锡214122 [2]江南大学分析测试中心,江苏无锡214122 [3]陕西省汉中市质检所,陕西汉中724000 |
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| 摘 要： | 通过衍生化反应,合成了AFB1羧甲基活化物,然后利用碳二亚胺法合成AFB1-O-BSA偶联物,构建AFB1完全抗原,并通过多种光谱和质谱对合成完全抗原过程中偶联比和结合位点进行研究。通过荧光光谱在分子水平上探讨AFB1与BSA载体蛋白的偶联机制及偶联反应对BSA的构象影响,推测黄曲霉毒素和牛血清白蛋白反应的结合部位,同时发生在BSA的酪氨酸残基和色氨酸残基上,使得BSA疏水性增加,肽链伸展程度降低。
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| 关 键 词： | 黄曲霉毒素B1 完全抗原构建 结合位点 |
Study on the Binding Site in the Construction of AFB1 Complete Antigen |
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| SUN Xiu-lan,ZHANG Yin-zhi,TANG Jian,ZHU Yu.Study on the Binding Site in the Construction of AFB1 Complete Antigen[J].Chinese Journal of Spine and Spinal Cord,2007(5). |
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| Authors: | SUN Xiu-lan ZHANG Yin-zhi TANG Jian ZHU Yu |
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| Institution: | 1. School of Food Science and Technology, Jiangnan University,Wuxi 214122 ,China; 2. Testing and Analysis Center, Jiang nan University, Wuxi 214122,China;3. Hanzhong Quality Supervision and Inspection Bureau, Hanzhong 724000,China |
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| Abstract: | To study the coupling ratio and the binding site of AFB1 and BSA during the construction of the AFB1 complete antigen, AFB1-carboxymethyl and AFB1-O-BSA complexation were synthesized by carbondiimide method. Different kind of spectroscopy and mass spectra |
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| Keywords: | aflationxin B1 immuno-antigen conjuation location |
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