树突状细胞在自身免疫性脑脊髓炎发病及耐受中作用的研究

目的　研究树突状细胞 ( dendritic cells,DCs)在实验性自身免疫性脑脊髓炎 ( experimental allergicencephalomyelitis,EAE)发病及免疫耐受中的作用。方法　诱导 EAE和口服耐受 EAE模型 ,采用流式细胞仪和直接免疫荧光法观察 EAE不同阶段、不同疾病程度外周淋巴结和中枢神经系统 ( CNS)中 DCs数量的变化。结果　免疫后第 4、7、9天外周淋巴结 DCs数量逐渐增多 ,第 9天脑、脊髓中开始有 DCs浸润 ,第 1 5天 DCs数量达高峰 ,第 1 5、2 3天 DCs数量与疾病症状严重程度相一致。口服耐受组与 EAE组差异无显著性。结论　外周淋巴结和 CNS局部 DCs在 EAE的发生、发展、转归尤其在触发发病中起重要作用。 DCs作为免疫反应的始动者 ,其数量变化在口服免疫耐受的产生机制中具次要作用

Studies on Dendritic Cells in the Onset and Oral Administrated Tolerance of Experimental Allergic Encephalomyelitis

Objective This study was designed to investigate DCs' role in the onset and oral administrated tolerance of experimental allergic encephalomyelitis (EAE). Methods Induction of EAE and oral tolerance. Immunofluorescence staining and flow cytometric analysis was selected, the numbers of DCs in different groups were measured. Results The number of DCs was increasing in peripheral lymph nodes on 4, 7, 9 d p.i. ( post inoculation), and reached the peak with the symptoms. DCs appeared in the spinal cords and brains on 9 d p.i. before EAE onset. There was little significant difference between oral group and EAE group, but there were significant differences among control group and clinical different symptom subgroups. Conclusions DCs located in peripheral lymph nodes and CNS, play an important role inEAEonset, developmentandrecovery. The number of DCs may play a secondary role in the mechanism of oral tolerance.