RP-HPLC法测定干姜、炮姜和生姜中3种姜酚的含量

目的:建立测定干姜、炮姜和生姜3种药用姜中6-姜酚、8-姜酚和10-姜酚含量的方法。方法:采用反相高效液相色谱法。色谱柱为依利特Hypersil ODS 2(250 mm×4.6 mm,5 m),流动相为乙腈-水(72∶28),流速为1 mL·min-1,检测波长为280 nm,柱温为25℃,进样量为10μL。结果:6-姜酚、8-姜酚和10-姜酚的进样浓度分别在1.156～231.200μg·mL-1(r=0.999 9)、1.072～214.400μg·mL-1(r=0.999 8)、1.031～206.200μg·mL-1(r=0.999 9)范围内与各自峰面积积分值呈良好线性关系;平均加样回收率分别为97.50%、98.06%、96.39%,RSD分别为1.18%、0.87%、1.37%(n均为6)。结论:本方法快速、准确、简便、高效,可为药用姜的质量控制和评价提供依据。

Content Determination of 3 Gingerols in Dried Ginger,Baked Ginger and Fresh Ginger by RP-HPLC

OBJECTIVE：To establish the method for the content determination of 6-gingerol,8-gingerol and 10-gingerol in 3 medicinal gingers including dried ginger,baked ginger and fresh ginger.METHODS：RP-HPLC method was adopted.Hypersil ODS 2（250 mm×4.6 mm,5 μm） column was used with acetonitrile-water（72：28） as mobile phase at a flow rate of 1 mL·min-1.The detection wavelength was set at 280nm and column temperature was 25 ℃ with the inject volume of 10 μL.RESULTS：The calibration curves of 6-gingerol,8-gingerol and 10-gingerol were linear in the range of 1.156～231.2 μg·mL-1（r=0.999 9）,1.072～214.4 μg·mL-1（r=0.999 8） and 1.031～206.2 μg·mL-1（r=0.999 9）,respectively.The average recoveries were 97.50%,98.06% and 96.39% with RSD at 1.18%,0.87% and 1.37%,respectively（n=6）.CONCLUSION：The method is rapid,accurate,simple and effective,which could provide reference for the quality control and evaluation of medicinal gingers.