小檗碱对异烟肼致肝损伤模型小鼠的保护作用及其机制研究

目的:观察小檗碱对异烟肼致肝损伤的保护作用及其机制。方法:NIH雄性小鼠30只,随机分为6组:正常对照组(生理盐水)、异烟肼组(180mg·kg-1)、联苯双酯组(异烟肼180mg·kg-1+联苯双酯150mg·kg-1)及小檗碱高、中、低剂量组(异烟肼180mg·kg-1+小檗碱100、50、25mg·kg-1),每日给药1次,连续5d。末次灌胃18h后处死小鼠,计算肝体指数,检测血清天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)活性及促炎因子白介素-6(IL-6)和抑炎因子IL-10含量,肝脏丙二醛(MDA)含量、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性。结果:与异烟肼组比较,小檗碱中、高剂量组肝体指数、AST、ALT、MDA、IL-6含量均显著降低(P<0.05或P<0.01),IL-10、SOD和GSH-Px水平显著升高(P<0.05或P<0.01)。结论:小檗碱可减轻异烟肼引起的肝损伤,其机制可能与抑制氧化应激和炎症反应有关。

Protection Mechanism of Berberine against Liver Injury Induced by Isoniazid in Mice

OBJECTIVE：To investigate the protective effects of berberine（Ber） against liver injury induced by isoniazid and its mechanism. METHODS：30 male mice were randomly divided into 6 groups：normal control group（normal saline） ,isoniazid group（180 mg·kg-1） ,bifendate group（isoniazid 180 mg·kg-1＋bifendate 150 mg·kg-1） ,Ber high-dose,medium-dose and low-dose groups（isoniazid 180 mg·kg-1＋Ber 100,50,25 mg·kg-1） . Mice were given medicine once a day for 5 days and sacrificed 18 h after the last intragastric administration. The hepatosomatic indices were calculated. The content of MDA,IL-6,IL-10 and the activities of ALT,AST,SOD and GSH-Px were detected. RESULTS：Compared with isoniazid group,the content of AST,ALT,MDA,IL-6 and hepatosomatic indices were decreased significantly（P0.05 or P0.01） ,and SOD,GSH-Px and IL-10 were increased significantly（P0.05 or P0.01） in Ber medium-dose and high-dose groups. CONCLUSION：Berberine has a protective effect on isoniazid-induced liver injury,the mechanisms may be related to inhibiting oxidative stress and inflammation.