| HPLC法测定多聚甲醛牙髓失活剂中盐酸可卡因含量 |
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| 引用本文： | 林瑶,杨敏茹,郭飞燕,王荣,王晓娟,顾宜.HPLC法测定多聚甲醛牙髓失活剂中盐酸可卡因含量[J].中国药房,2011(25):2372-2373. |
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| 作者姓名： | 林瑶 杨敏茹 郭飞燕 王荣 王晓娟 顾宜 |
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| 作者单位： | 第四军医大学第三附属医院药剂科;陕西省新药评审中心; |
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| 摘 要： | 目的:建立测定多聚甲醛牙髓失活剂中盐酸可卡因含量的方法。方法:采用高效液相色谱法。色谱柱为Diamonsil C18,流动相为甲醇-0.5%三乙胺溶液(磷酸调pH值至4.5)=30∶70,检测波长为235nm,流速为1.0mL·min-1,柱温为25℃,进样量为10μL。结果:盐酸可卡因检测浓度线性范围为16.208～129.664μg·mL-1(r=0.9997);平均加样回收率为95.12%,RSD=1.15%。结论:所建立的方法简便、灵敏、快速,结果准确、可靠。
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| 关 键 词： | 多聚甲醛牙髓失活剂 盐酸可卡因 高效液相色谱法 含量 |
Content Determination of Cocaine Hydrochloride in Paraformaldehyde Dental Pulp Devitalizing Agent by HPLC |
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| LIN Yao,GUO Fei-yan,WANG Rong,WANG Xiao-juan,GU Yi YANG Min-ru.Content Determination of Cocaine Hydrochloride in Paraformaldehyde Dental Pulp Devitalizing Agent by HPLC[J].China Pharmacy,2011(25):2372-2373. |
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| Authors: | LIN Yao GUO Fei-yan WANG Rong WANG Xiao-juan GU Yi YANG Min-ru |
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| Institution: | (The Third Affiliated Hospital of Fourth Military Medical University,Xi’an 710032,China) YANG Min-ru(Shaanxi Provincial New Medicine Evaluation Center,Xi’an 710065,China) |
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| Abstract: | OBJECTIVE： To establish a method for the content determination of cocaine hydrochloride in Paraformaldehyde dental pulp devitalizing agent.METHODS： HPLC method was adopted.The determination was performed on Diamonsil-C18 column with mobile phase consisted of methanol -0.5% triethylamine （pH adjusted to 4.5 using phosphoric acid） （30 ∶ 70） at flow rate of 1.0 mL·min-1.The detection wavelength was set at 235 nm and the column temperature was 25 ℃.The sample size was 10 μL.RESULTS： The linear range of cocaine hydrochloride was 16.208～129.664 μg·mL-1 （r=0.999 7）,the average recovery was 95.12%（RSD=1.15%）.CONCLUSION： The method is simple,sensitive,rapid,accurate and reliable. |
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| Keywords: | Paraformaldehyde dental pulp devitalizing agent Cocaine hydrochloride HPLC Content |
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