| HPLC法测定木薯叶中芦丁的含量 |
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| 引用本文： | 何翠薇,覃洁萍,黄俏妮.HPLC法测定木薯叶中芦丁的含量[J].中国药房,2011(23):2160-2161. |
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| 作者姓名： | 何翠薇 覃洁萍 黄俏妮 |
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| 作者单位： | 广西中医学院; |
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| 基金项目： | 广西中医学院自然科学研究课题(P2009030) |
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| 摘 要： | 目的:建立测定木薯叶中芦丁含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent ZORBAX Eclipse XDB C18(0.105006～mm0.×046.06mmgm.,m5Lμ-1m范)围,流内动与相峰为面甲积醇积-分0.1值%呈磷良酸好(1的2:线88性),关流系速(为r=10.m99L.9m9)i;n-平1,均检加测样波回长收为率35为4n1m00。.7结2%果,:R芦SD丁=的2.检3%测(浓n=度6)在。结论:本方法操作简便、准确、重复性好,可作为木薯叶中芦丁的含量测定方法。
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| 关 键 词： | 高效液相色谱法 木薯叶 芦丁 含量测定 |
Content Determination of Rutin in Manihot esculenta Leaves by HPLC |
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| HE Cui-wei,QIN Jie-ping,HUANG Qiao-ni.Content Determination of Rutin in Manihot esculenta Leaves by HPLC[J].China Pharmacy,2011(23):2160-2161. |
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| Authors: | HE Cui-wei QIN Jie-ping HUANG Qiao-ni |
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| Institution: | HE Cui-wei,QIN Jie-ping,HUANG Qiao-ni(Guangxi College of Traditional Chinese Medicine,Nanning 530001,China) |
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| Abstract: | OBJECTIVE：To establish a method for the content determination of rutin in Manihot esculenta leaves.METHODS：HPLC method was adopted.Agilent ZORBAX Eclipse XDB C18（150 mm×4.6 mm,5 μm） column was used with mobile phase consisted of methanol-0.1% phosphoric acid（12：88） at flow rate of 1 mL·min-1.The detection wavelength was set at 354 nm.RESULTS：The linear range of rutin was 0.006～0.060 mg·mL-1（r=0.999 9） with an average recovery of 100.72%（RSD=2.3%,n=6）.CONCLUSION：The method is simple,accurate,reproducible and could be used for the content determination of rutin in M.esculenta leaves. |
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| Keywords: | HPLC Manihot esculenta leaves Rutin Content determination |
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