红外光辅助萃取-HPLC法测定玄参中肉桂酸和哈巴俄苷的含量

目的:建立测定玄参中肉桂酸和哈巴俄苷含量的方法。方法:采用红外光辅助萃取-高效液相色谱法。色谱柱为Diamonsil C18(200mm×4.6mm,5μm),流动相为1%乙酸溶液-甲醇(55∶45),柱温为25℃,流速为1mL·min-1,检测波长为278nm,以外标法计算含量。红外光辅助萃取条件的溶剂为甲醇-水(50∶50),提取时间为5min。结果:肉桂酸和哈巴俄苷的检测浓度均在5～100μg·mL-1范围内与峰面积积分值呈良好线性关系(r均为0.9999);二者平均回收率分别为101.7%和103.2%,RSD分别为0.71%和0.93%(n=6)。结论:本方法简便、准确,适用于分析测定玄参中肉桂酸和哈巴俄苷的含量。

Content Determination of Cinnamic Acid and Harpagoside in Scrophularia ningpoensis by Infrared Heating Assisted Extraction-HPLC

OBJECTIVE：To develop a method for the content determination of cinnamic acid and harpagoside in Scrophularia ningpoensis. METHODS：Infrared heating assisted extraction-HPLC（IHAE-HPLC） was adopted. The determination was performed on Diamonsil C18（200 mm×4.6 mm,5 μm） column with 1% acetic acid solution-methanol（55 ∶ 45） as mobile phase at flow rate of 1 mL·min-1. The column temperature was set at 25 ℃. And the detection wavelength was 278 nm. The contents of cinnamic acid and harpagoside were calculated using external standard method. Cinnamic acid and harpagoside was extracted by IHAE-HPLC using methanol-water（50 ∶ 50） as solvent with extraction time of 5 min. RESULTS：The linear range for cinnamic acid and harpagoside were 5～100 μg·mL-1（r=0.999 9） . The average recoveries of cinnamic acid and harpagoside were 101.7%（RSD=0.71%,n=6） and 103.2%（RSD=0.93%,n=6） . CONCLUSION：The method is simple and accurate. It is adapted to determine and analyze the content of cinnamic acid and harpagoside in S. ningpoensis.