| RP-HPLC法测定清肺抑火丸中芒果苷的含量 |
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| 引用本文： | 张丽珍,周之荣. RP-HPLC法测定清肺抑火丸中芒果苷的含量[J]. 中国药房, 2011, 0(24): 2294-2295 |
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| 作者姓名： | 张丽珍 周之荣 |
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| 作者单位： | 广东药学院; |
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| 摘 要： | 目的:建立清肺抑火丸中芒果苷的含量测定方法。方法:采用反相高效液相色谱法。色谱柱为Diamonsil-C18柱,流动相度为在乙腈5.1-0～.25%1.0磷μ酸g·水m溶L-液1范(围13内∶8与7)峰,流面速积为积1分.0值m呈L·良m好in-线1,性检关测系波(长r=为02.95989n7m),;进芒样果量苷为平均10加μL样,回柱收温率为为359℃9.6。%结,R果S:D芒=果0.苷54检%(测n=浓6)。结论:该法精密度、稳定性、加样回收率和重复性均能满足定量的要求,可用于清肺抑火丸的质量控制。
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| 关 键 词： | 清肺抑火丸 芒果苷 反相高效液相色谱法 含量测定 |
Content Determination of Mangiferin in Qingfei Yihuo Pills by RP-HPLC |
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| ZHANG Li-zhen,ZHOU Zhi-rong. Content Determination of Mangiferin in Qingfei Yihuo Pills by RP-HPLC[J]. China Pharmacy, 2011, 0(24): 2294-2295 |
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| Authors: | ZHANG Li-zhen ZHOU Zhi-rong |
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| Affiliation: | ZHANG Li-zhen,ZHOU Zhi-rong(Guangdong Pharmaceutical University,Guangzhou 510006,China) |
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| Abstract: | OBJECTIVE： To establish the method for the content determination of mangiferin in Qingfei yihuo pills. METHODS： RP-HPLC method was adopted. The chromatographic column was performed on Diamonsil-C18 column. The mobile phase was consisted of acetonitrile-0.2% phosphoric acid（13 ∶ 87）. The flow rate was 1.0 mL·min-1 and the detection wavelength was 258 nm. The injection volume was 10 μL and the column temperature was 35 ℃. RESULTS： The linear range of mangiferin was 5.1～51.0 μg·mL-1（r=0.999 7）with an average recovery of 99.6 % （RSD=0.54%, n=6）. CONCLUSION： The precision, stability, recovery and reproducibility of the method are up to the requirement of quantitative determination. It can be used for content determination of Qingfei yihuo pills. |
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| Keywords: | Qingfei yihuo pills Mangiferin RP-HPLC Content determination |
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