| HPLC法同时测定黄海棠中山柰酚和槲皮素的含量 |
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| 引用本文： | 张伟,于海林,宋艳丽,康文艺.HPLC法同时测定黄海棠中山柰酚和槲皮素的含量[J].中国药房,2011(19):1790-1792. |
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| 作者姓名： | 张伟 于海林 宋艳丽 康文艺 |
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| 作者单位： | 黄河科技学院;河南大学中药研究所; |
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| 基金项目： | 河南省科技厅重点攻关项目(102102310019) |
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| 摘 要： | 目的:建立同时测定黄海棠中山柰酚和槲皮素含量的方法。方法:采用高效液相色谱法。色谱柱为Purospher Star RP-C18(250mm×4.6mm,5μm),流动相为甲醇-0.025mo·lL-1磷酸水溶液(60∶40),流速为1.0mL·min-1,柱温为25℃,检测波长为370nm。结果:山柰酚、槲皮素进样量的线性范围分别为0.0184～0.1288μg(r=0.9998)、0.008～0.064μg(r=0.9999);二者的平均加样回收率分别为97.12%(RSD=1.03%,n=6)、96.51%(RSD=1.32%,n=6)。结论:本方法灵敏、准确、重复性好,可用于黄海棠的质量控制。
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| 关 键 词： | 黄海棠 高效液相色谱法 山柰酚 槲皮素 含量测定 |
Content Determination of Kaempferol and Quercetin in Hypericum ascyron by HPLC |
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| ZHANG Wei ZHANG Wei,YU Hai-lin,SONG Yan-li,KANG Wen-yi.Content Determination of Kaempferol and Quercetin in Hypericum ascyron by HPLC[J].China Pharmacy,2011(19):1790-1792. |
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| Authors: | ZHANG Wei ZHANG Wei YU Hai-lin SONG Yan-li KANG Wen-yi |
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| Institution: | ZHANG Wei(Huanghe College of Science and Technology,Zhengzhou 450063,China) ZHANG Wei,YU Hai-lin,SONG Yan-li,KANG Wen-yi(Institute of Chinese Materia Medica,Henan University,Kaifeng 475004,China) |
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| Abstract: | OBJECITVE：To establish the method for the content determination of kaempferol and quercetin in Hypericum ascyron. METHODS：HPLC method was adopted. The separation was performed on Purospher Star RP-C18（250 mm×4.6 mm,5 μm） column with mobile phase consisted of methanol-0.025 mol·L-1 phosphate（60 ∶ 40） at flow rate of 1.0 mL·min-1. The column temperature was 25℃ and determination wavelength was set at 370 nm. RESULTS：The linear ranges of kaempferol and quercetin was 0.018 4～0.128 8 μg（r=0.999 8） and 0.008～0.064 μg（r=0.999 9） . The average recoveries were 97.12%（RSD=1.03%,n=6） and 96.51%（RSD=1.32%,n=6） . CONCLUSION：The method is sensitive,accurate,and reproducible for the simultaneous determination of kaempferol and quercetin in H. ascyron. |
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| Keywords: | Hypericum ascyron HPLC Kaempferol Quercetin Content determination |
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