| 异甘草素固体脂质纳米粒包封率测定方法的研究 |
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| 引用本文： | 陈卫军,袁勇,王鲁妹,智永刚. 异甘草素固体脂质纳米粒包封率测定方法的研究[J]. 中国药房, 2011, 0(21): 1994-1995 |
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| 作者姓名： | 陈卫军 袁勇 王鲁妹 智永刚 |
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| 作者单位： | 石河子大学医学院第一附属医院;石河子大学药学院; |
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| 摘 要： | 目的:建立异甘草素固体脂质纳米粒(ISO-SLN)包封率测定方法。方法:采用透析法分离样品中的游离药物及载药固体脂质纳米粒,并确定透析介质和透析时间;紫外分光光度法测定包封率,检测波长为396nm。结果:以2%泊洛沙姆188水溶液为透析介质、透析时间为8h,能有效分离ISO-SLN与游离ISO,加样回收率大于95%;ISO检测浓度线性范围为1.12～7.84μg·mL-1(r=0.9995),低、中、高浓度平均回收率为99.54%、99.11%、100.2%,RSD均小于5%;3批ISO-SLN平均包封率为80.2%。结论:所建立的方法可用于测定ISO-SLN包封率,且方法准确、简便。
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| 关 键 词： | 异甘草素 固体脂质纳米粒 包封率 透析法 紫外分光光度法 |
Determination Method of Encapsulation Efficiency of Isoliquiritigenin Solid Lipid Nanoparticles |
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| CHEN Wei-jun,YUAN Yong,WANG Lu-mei,ZHI Yong-gang YUAN Yong. Determination Method of Encapsulation Efficiency of Isoliquiritigenin Solid Lipid Nanoparticles[J]. China Pharmacy, 2011, 0(21): 1994-1995 |
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| Authors: | CHEN Wei-jun YUAN Yong WANG Lu-mei ZHI Yong-gang YUAN Yong |
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| Affiliation: | CHEN Wei-jun,YUAN Yong,WANG Lu-mei,ZHI Yong-gang(The First Affiliated Hospital of Medical College,Shihezi University,Shihezi 832008,China) YUAN Yong(Pharmacy College of Shihezi University,Shihezi 832002,China) |
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| Abstract: | OBJECTIVE:To establish the method for encapsulation efficiency determination of Isoliquiritigenin solid lipid nanoparticles(ISO-SLN) .METHODS:Dialysis method was used to isolate free fraction and drug-loading solid lipid nanoparticles from samples.Medium and time of dialysis were confirmed.The encapsulation efficiency was determined by UV spectrophotometry at detection wavelength of 396 nm.RESULTS:2% poloxamer188 hydrate medium was used as dialysis medium and dialysis time was 8 h.Free ISO could be effectiv... |
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| Keywords: | Isoliquiritigenin Solid lipid nanoparticles Encapsulation efficiency Dialysis method UV spectrophotometry |
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