| 人免疫缺陷病毒1型(HIV-1)gag基因疫苗的免疫原性 |
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| 引用本文： | 江文正,金宁一,李子健,张立树.人免疫缺陷病毒1型(HIV-1)gag基因疫苗的免疫原性[J].细胞与分子免疫学杂志,2004,20(3):272-273. |
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| 作者姓名： | 江文正 金宁一 李子健 张立树 |
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| 作者单位： | 1. 解放军军需大学全军基因工程重点实验室,吉林,长春,130062
2. 吉林大学第一医院,吉林,长春,130023 |
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| 基金项目： | 国家杰出青年科学基金资助 (No.3982 51 1 9)，国家高技术研究发展计划 (863)资助项目(No.2 0 0 1AA2 1 50 31 ) |
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| 摘 要： | 目的 :检测人免疫缺陷病毒 1型 (HIV 1)gag基因疫苗的免疫原性。方法 :分别以ELISA、荧光抗体染色和乳酸脱氢酶释放法 ,检测免疫小鼠血清抗体滴度、脾T细胞亚群的数量和淋巴细胞杀伤效应。结果 :血清抗体滴度、脾T细胞亚群的数量及淋巴细胞杀伤效应 ,重组质粒pVAXGAG免疫组与空载体pVAX1对照组相比较差异显著(分别为P <0 .0 5和P <0 .0 1)。结论 :HIV 1DNA疫苗质粒pVAXGAG在BALB/c小鼠中不仅可诱导特异性体液免疫 ,而且可诱导特异性细胞免疫。
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| 关 键 词： | 人免疫缺陷病毒1型 gag 核酸疫苗 免疫原性 |
| 文章编号： | 1007-8738(2004)03-0272-02 |
| 修稿时间： | 2003年6月18日 |
Study on the immunogenicity of HIV-1 gag vaccine |
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| JIANG Wen zheng ,JIN Ning yi ,LI Zi jian ,ZHANG Li shu Key Laboratory of Genetic Engineering,Quartermaster University of PLA,Changchun , First Hospital,Jilin University,Changchun ,China.Study on the immunogenicity of HIV-1 gag vaccine[J].Journal of Cellular and Molecular Immunology,2004,20(3):272-273. |
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| Authors: | JIANG Wen zheng JIN Ning yi LI Zi jian ZHANG Li shu Key Laboratory of Genetic Engineering Quartermaster University of PLA Changchun First Hospital Jilin University Changchun China |
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| Institution: | Key Laboratory of Genetic Engineering, Quartermaster University of PLA, Changchun 130062, China. wzjiang@hotmail.com |
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| Abstract: | AIM: To detect the immunogenicity of HIV-1 gag DNA vaccine. METHODS: The serum antibody titers of the immunized mice were detected by ELISA. The numbers of spleen's CD4(+) and CD8(+) T cells were analyzed by the fluorescent antibody staining and the cytotoxic activity was determined by lactate dehydrogenase (LDH) release assay. RESULTS: The titers of the serum antibodies and the numbers and cytotoxic activity of spleen's T lymphocyte subsets had significant differences (P<0.05 and P<0.01, respectively) between pVAXGAG and pVAX1 vaccinated groups. CONCLUSION: HIV-1 DNA vaccine plasmid pVAXGAG could elicit both the specific humoral and cellular immunities in BALB/c mice. |
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| Keywords: | HIV-1 gag DNA vaccine immunogenicity |
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