| Annexin A1在小鼠胚泡植入不同时期子宫内膜组织中的表达及意义 |
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| 引用本文： | 张群,刘学庆,陈雪梅,王应雄,赖雪梅,何俊琳. Annexin A1在小鼠胚泡植入不同时期子宫内膜组织中的表达及意义[J]. 生殖与避孕, 2008, 28(5): 257-263 |
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| 作者姓名： | 张群 刘学庆 陈雪梅 王应雄 赖雪梅 何俊琳 |
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| 作者单位： | [1]重庆医科大学公共卫生学院生殖生物学研究室,重庆400016 [2]重庆医科大学临床检验诊断学省部共建教育部重点实验室,重庆400016 |
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| 摘 要： | 目的:探讨Annexin A1在小鼠胚泡植入期的作用。方法:双向聚丙烯酰胺凝胶电泳(2D-PAGE)分析孕d3、d5、d7小鼠子宫内膜蛋白质组,用差异蛋白质组学显示pI约5.1、分子量约38kD的蛋白点在d3、d5上调,且在d5更明显。经基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)测定其胶内酶解后的肽质量指纹谱(peptide mass fingerprint,PMF)和数据库搜索对此蛋白点进行鉴定;再用RT-PCR、原位杂交、Western blot和免疫组织化学技术进行验证分析。结果:该蛋白点鉴定为鼠源性Annexin A1;d5小鼠子宫内膜组织中Annexin A1的mRNA和蛋白质水平均增加。结论:Annexin A1可能参与胚泡着床这一重要生命活动过程。
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| 关 键 词： | Annexin A1 胚泡植入 蛋白质组 小鼠 子宫内膜 |
Expression of Annexin A1 in Endometrium during the Blastocyst Implantation in Mouse |
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| Abstract: | Objective: To investigate the role of Annexin A1 during the blastocyst implantation of mouse. Methods: Total proteins of mice endometria from pregnant d 3,d 5 and d 7 were analyzed by two-dimensional gel electrophoresis and image analysis,respectively. Differential expression protein spots were excised by dot-gel digestion with trypsin. Then peptide mass fingerprinting (PMF) was submitted to an appropriate software (Mascot: PMF) for identifying proteins. A differential protein spot was checked by RT-PCR,Western blotting,immunohistochemistry and in-situ hybridization. Results: The differential protein spot was identified as Annexin A1 by PMF. The expression level of Annexin A1 mRNA and protein reached the maximum at the d 5 of pregnant. Conclusion: Annexin A1 might play an important role during the blastocysti mplantation. |
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| Keywords: | Annexin A1 blastocyst implantation proteome mouse endometrium |
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