黄珠子草有效成分短叶苏木酚及8,9-单环氧短叶苏木酚对大鼠肝损伤的保护作用

目的:探讨黄珠子草有效成分短叶苏木酚及8,9-单环氧短叶苏木酚保肝作用及机制。方法:SD大鼠ip四氯化碳(CCl ₄)或ig乙醇造成急性和慢性肝损伤,比色分析法检测血清丙氨酸氨基转移酶(ALT)、门冬氨酸转移酶(AST)和总胆红素(TB IL)水平评价肝功能;分别用硫代巴比妥酸比色法和黄嘌呤氧化酶法测定血清、肝脏组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,并观察对血液流变学参数的影响。结果:短叶苏木酚和8,9-单环氧短叶苏木酚对CC l₄致ALT升高均有降低作用,对AST升高无明显影响;亦可拮抗乙醇诱致的大鼠血清TB IL的病理性升高;对肝损伤大鼠血清和肝脏MDA的升高、SOD活性的降低呈现出不同的改善作用;降低异常升高的血液流变学各项指标。结论:黄珠子草有效成分短叶苏木酚及8,9-单环氧短叶苏木酚对急慢性肝损伤具有保护作用,其机制与对抗自由基脂质过氧化及改善血液循环有关。

Protecting effect of brevifolin and 8,9-single-epoxy brevifolin of Phyllanthus simplex on rat liver injury

Objective: To investigate the effect of protecting liver of brevifolin and 8,9-single-epoxy brevifolin of Phyllanthus simplex.Method: Rats were administered with CCl₄(ip) or alcohol(ig) to establish acute or chronic liver injured model,respectively.ALT,AST and TBIL in serum were measured using colorimetric analysis to evaluate liver function.MDA content or SOD activity in serum and liver tissue was measured by thiobarbituric acid chromatometry and xanthine oxidase methods,respectively.The hemorheological parameters were observed.Result: Brevifolin and 8,9-single-epoxy brevifolin reduced the increase of ALT induced by CCl₄,but they did not influence the increase of AST.And it could inhibit the pathologic increase of serum TBIL induced by alcohol.They could ameliorate the MDA increase or SOD decrease in serum and liver tissue in rats with liver injury,and decrease abnormal changed hemorheological parameters.Conclusion: Brevifolin and 8,9-single-epoxy brevifolin show protective effective against acute and chronic liver injuries,and the mechanism is relevant to antagonizing the lipid peroxidation of free radical and improving the blood circulation.