复方萘酚喹对伯氏疟原虫ANKA株红内斯超微结构的影响

为观察复方萘酚喹及其各单药 (磷酸萘酚哇、双氢青蒿素、甲氧苄啶 )对伯氏疟原虫ANKA株红内期超微结构的影响 ,将感染伯氏疟原虫ANKA株的小鼠分别以复方萘酚喹 2 6 0mg·kg- 1·d- 1× 1d (内含磷酸萘酚喹 149mg、双氢青蒿素 37mg、甲氧苄啶 74mg) ,磷酸萘酚喹 149mg·kg- 1·d- 1× 1d ,双氢青蒿素 180mg·kg- 1·d- 1× 1d和甲氧苄啶 1152mg·kg- 1·d- 1× 1d灌胃给药。给药后 1、 2、 4、 8、 12和 2 4h ,采血作标本 ,经包埋 ,切片 ,染色后 ,用电镜镜检。结果 :复方萘酚喹于给药 2h后多数晚期滋养体出现质膜肿胀、破裂 ,有的呈空腔状 ;食物泡膜肿胀 ;线粒体膜肿胀、分层、剥离 ;色素颗粒变形。给药 4h后进一步加剧。 8h后小部分晚期滋养体结构已经融化崩解 ,内容物结构模糊。 12h后绝大多数晚期滋养体结构已完全破坏或消失。结论 :复方萘酚喹对伯氏疟原虫红内期超微结构的影响较其组分中各单药起效更快、力度更强和作用相对彻底。

Effect of Compound Naphthoquine on the Ultrastructure of Plasmodium berghei ANKA Strain in Erythrocytic Stage

Objective:To observe the effect of compound naphthoquine on the ultrastructure of erythrocytic stage of plasmodlum berghei ANKA strain and compare with naphthoquine phosphate, dihydroartemisinin and trimethoprim.Methods:NIH mice were infected with plasmodium berghei ANKA strain and administered ig with compound naphthoquine, dihydroartemisinin, trimethoprim and naphthoquine phosphate respectively. Blood samples were collected at 1st,2nd,4th,8th,12th and 24th hour after drug administration, and the ultra-thin sections were mounted, stanined and examined under electron microscope. Results:The effect of compound naphthoquine on the ultrastructure of erythrocytic stage of P. berghei ANKA strain demonstrated that there is a potent and rapid lethal action on the late trophozoites of erythrocytic forms by interfering the physiological function of membranous phase system. After 2h medication, most of late trophozoites showed progressive morphological changes such as swelling, breaking and vacuolated, the interspace appeared with fusion of food vacuoles, the mitochondrial membranes swollen, separated and stripped and the malaria pigments deformed. After 4h exposure to compound naphthoquine,the degenerated parasites increased. After 12h medication,the structure of most of late trophozoites was destroyed.Conclusion:The effect of compound naphthoquine on the ultrastructure of erythrocytic stage of plasmodium berghei ANKA strain is more prompt and stronger than other relevant drugs.