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Visualization of actin fibers associated with the cell membrane in amoebae of Dictyostelium discoideum. |
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| Authors: | M Clarke G Schatten D Mazia J A Spudich |
| Abstract: | Amoebae of Dictyostelium discoideum were attached to a surface coated with polylysine, and the upper portion of the cells was sheared off with a stream of buffer. Scanning and transmission electron microscopy showed that the cytoplasmic surface of the exposed membrane was covered with fibers consisting of actin-containing filaments. The actin was identified by its solubility properties and its ability to interact with muscle myosin. |
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