深低温停循环逆行脑灌注脑组织自由基变化的实验研究

研究深低温停循环（ＤＨＣＡ）与逆行脑灌注（ＲＣＰ）时脑组织自由基的变化。健康杂种犬１４只，随机等分为ＤＨＣＡ组和ＲＣＰ组，在停循环前（Ａ点）、ＤＨＣＡ／ＲＣＰ３０分（Ｂ点）、ＤＨＣＡ／ＲＣＰ６０分（Ｃ点）ＤＨＣＡ／ＲＣＰ９０分（Ｄ眯）和复温再灌注３０分（Ｅ点）取脑皮层ｌｇ，检测丙二醛（ＭＤＡ）和超氧化物歧化酶（ＳＯＤ）水平。结果见两组在Ａ点ＭＤＡ和ＳＯＤ无差别。在Ｂ、Ｃ、Ｄ、Ｅ点，ＤＨＣＡ组ＭＤＡ

Experimental Study on Free Radical Changes in Cerebral Tissue during Deep Hypothermic Circulation Arrest and Retrograde Cerebral Perfusion

Aim:To assess the effect of retrograde cerebral perfusion(RCP) on brain protection during deep hypothermic circulation arrest (DHCA).Method:Fourteen dogs were randomly divided into control group (n=7) and experimental group (n=7).DHCA was employed in both control and experimental groups.RCP was performed only in experimental group.Specimens were obtained from the cerebral tissue to measure MDA and SOD at five different time intervals:before DHCA,30,60 and 90 minutes after DHCA RCP,and 30 minutes after reperfusion.Result:Before DHCA,no significant difference,in the levels of MDA and SOD was found between two groups.After DHCA RCP,however,the MDA level in control group was gradually increased and SOD decreased,but there was no significant change in experimental group.At the same time intervals,there was significant difference between two groups.Conclusion:Oxygen free radical is an important factor in brain damage after DHCA.RCP could be a new approach to maintain blood supply to brain tissue during DHCA,so asto minimize or eliminate free radical reaction and reduce the risk of brain injury.