nm23及NDRG1 蛋白在卵巢癌肿瘤抑郁模型中的表达*

目的:探讨不良心理应激影响肿瘤发生发展的分子学机制。方法:采用蛋白组学方法筛选、鉴定荷卵巢癌裸鼠不良心理应激模型移植瘤中的差异表达蛋白，并用Western Blot验证；用免疫组化SP法检测25例应激组和20例对照组移植瘤中的nm23及NDRG 1 蛋白的表达特点。结果:两组在pI 7.1、Mr=21ku和pI 5.5、Mr=43ku处有两个显著差异点，前一个在应激组表达上调而后一个表达下调，通过质谱分别鉴定为nm23及NDRG 1 蛋白；Western Blot验证结果同蛋白组学的一致；免疫组化显示nm23蛋白在应激组和对照组中阳性表达率分别为100% 和70% ，NDRG 1 蛋白为56% 和85% ，差异均有统计学意义（P<0.05）。 结论:不良心理应激可能通过激活癌基因和失活抑癌基因来影响肿瘤的发生发展，nm23及NDRG 1 可作为基因研究和基因治疗的靶点。 

Expression of nm23 and NDRG1 Proteins in Ovarian Cancer Tumor Model of Depression

Objective: To study the molecular mechanisms of adverse psychological stress on ovarian cancer tumori -genesis. Methods:proteomics methodwas employed to locate and identify differentially expressed proteins in ovarian can-cer-bearing nude mice xenograft model of adverse psychological stress, to be verified using Western Blot. The expression of nm23protein and NDRG1 protein were detected by an immunohistochemical SP method in transplanted tumor tissue in-cluding 25cases in the stress group and 20cases in the control group. Results: At pI7.1, Mr = 21ku, and pI 5.5, Mr = 43 ku, there are two notable points of difference, the previous expression was increased in the stress group while another had decreased expression, they were identified as nm23protein and NDRG1 protein by mass spectrometry; the Western Blot-ting results were consistent with those of proteomics method; and the results of immunohistochemistry showed that the pos -itive rate of nm 23in the stress group and control group were 100 % and 70%, respectively, while rates of NDRG1 protein were56% and 85%, respectively, the differences were significant (P<0.05). Conclusion:Adverse psychological stress may influence the development of tumors by activation of oncogenes and inactivation of tumor suppressor genes, and nm 23 and NDRG 1 could be used as a genetic research and gene therapy target.