Fmrl基因敲除小鼠血清雌性激素含量的比较研究

目的测定不同周龄和超排前后雌性Fmrl基因敲除FVB小鼠和背景FVB小鼠血清生殖激素雌二醇（R）、孕酮（P）、促黄体生成激素（LH）和促卵泡生成激素（FSH）含量，比较不同周龄和不同状态下敲除基因动物的血清性激素水平，研究敲除基因对动物的发育和生殖生理等方面的影响。方法用放射免疫分析法测定上述动物不同周龄和超排前后血清E2、P、LH和FSH的含量，测定Fmrl基因敲除小鼠不同周龄时的超排卵数，进行统计学分析。结果6周龄的雌性Fmrl基因敲除小鼠与FVB小鼠比较，LH、FSH值差异无显著性（P〉0．05），E2的P值差异有显著性（P〈0．05）。lO周龄的两组动物间血清生殖激素水平和超排卵数均差异无显著性（P〉0．05）。lO周龄Fmrl基因敲除小鼠的超排卵数与6周龄动物比较差异有显著性（P〈0．05）。结论敲除Fmrl基因对动物雌性激素的分泌有影响，该基因与动物的生殖生理有一定的关联。

FSH, LH, E2 and P in Blood Serum of Fmrl Knockout Mouse and FVB Mouse

Objective To assess and analyze the levels of estradial(E_2),progesterone(P),follicle-stimulating hormone(FSH) and luteinizing hormone(LH) in blood serum of Fmr1 knockout mice and FVB mice before and after superovulation.Method The levels of E_2,P,FSH and LH were measured by RIA and statistically analyzed.Results The levels of FSH and LH were not different between Fmr1 knockout mice and FVB mice.The levels of E_2 and P were higher in FVB mice(P<0.01).The levels of E_2,P,FSH,LH and the amount of ova had no significant difference between Fmr1 knockout mice and FVB mice at 10 weeks of age after superovulation(P>0.05),but the amount of ova of superovulated Fmr1 knockout mice 10 weeks old was higher than those in 6 weeks old(P>0.05).Conclusion Fmr1 gene may perhaps influence the E_2,P,FSH and LH levels in serum.It should be taken into consideration in mouse reproductive physiological studies.