HPLC法测定白芍和赤芍中1,2,3,4,6-五-O-倍酰-D-葡萄糖含量

目的用高效液相色谱(HPLC)法测定白芍和赤芍中1,2,3,4,6-五-O-倍酰-D-葡萄糖(PGG)的含量。方法用50%乙醇水溶液,通过超声提取法从白芍和赤芍中提取PGG。采用Diamonsil C18色谱柱,流动相为乙腈-2.5%醋酸水溶液(18∶82,V/V),pH为2.85,紫外检测波长为280nm,流速为1mL/min。结果PGG在5μg/mL~150μg/mL范围内呈良好的线性关系(r=0.9999,n=5),平均回收率为100.9%,RSD为3.57%(n=9)。在白芍和赤芍中PGG的含量分别为2.48mg/g和2.24mg/g。结论本实验所建立的测定PGG含量的HPLC法能够有效消除白芍和赤芍中其他组分的干扰,操作简单快速,精密度高、重现性好、回收率高,适用于中药提取物中PGG含量的测定。

Determination of 1,2,3,4,6-penta-O-galloyl-D-glucose in Radix Paeoniae Alba and Radix Paeoniae Rubra with HPLC

Objective In recent reports,it has been indicated that 1,2,3,4,6-penta-O-galloyl-D-glucose(PGG)in Chinese herbal medicines is a natural active component which exhibited distinct anticancer,antidiabete and anti-obesity activities.A rapid,sensitive and reliable method using high-performance liquid chromatography is to be developed for determination of PGG in Radix Paeoniae Alba and Radix Paeoniae Rubra.Methods PGG was extracted with 50% ethanol from Radix Paeoniae Alba and Radix Paeoniae Rubra.For the chromat...