| 应用40条引物进行不同品系小鼠RAPD遗传检测的初步研究 |
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| 引用本文： | 吴开平,魏泓. 应用40条引物进行不同品系小鼠RAPD遗传检测的初步研究[J]. 第三军医大学学报, 2000, 22(1): 72-74 |
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| 作者姓名： | 吴开平 魏泓 |
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| 作者单位： | 第三军医大学实验动物中心,重庆,400038 |
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| 基金项目： | 国家自然科学基金!39600079，全军“九五”医药卫生科研基金!96M038 |
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| 摘 要： | 目的：探讨运用ＲＡＰＤ－ＰＣＲ对小鼠进行遗传检测的可能性，筛选在各品系小鼠间具有多态性的引物。方法：运用４０条引物对ＴＡ１、ＢＡＬＢ／ｃ、ＮＩＨ、ＳＣＩＤ、Ｔ７３９、ＤＢＡ／２、６１５、ＢＡＬＢ／ｃ－ｎｕ／ｎｕ等９个品系小鼠的ＤＮＡ进行ＲＡＰＤ－ＰＣＲ扩增，以琼脂糖凝胶电泳观察结果。结果：４０条引物中只筛选出２条多态性引物，其它３８条引物扩增结果无品系间多态性。结论：ＲＡＰＤ－ＰＣＲ方法可以区分９
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| 关 键 词： | 遗传检测 小鼠 RAPD－PCR 多态性 |
Preliminary study of genetic monitoring of various strains of mouse with RAPD-PCR using 40 single primers |
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| WU Kai-ping,WEI Hong. Preliminary study of genetic monitoring of various strains of mouse with RAPD-PCR using 40 single primers[J]. Acta Academiae Medicinae Militaris Tertiae, 2000, 22(1): 72-74 |
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| Authors: | WU Kai-ping WEI Hong |
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| Abstract: | Objective: To assess the possibility of genetic monitoring of various strains of mouse with RAPD PCR using 40 single primers. Methods: The DNA of 9 strains of mouse including TA1, BALB/c, KM, NIH, SCID, T739, DBA/2, 615 and BALB/c nu/nu was amplified with RAPD PCR using 40 single primers. The PCR products were resolved with electrophoresis on agarose gel and stained with ethidium bromide. Results: 2 of the 40 single primers resulted in high polymorphism and all the other primers produced monomorphic amplification products in 9 mouse strains. Conclusion: RAPD PCR is an effective technique to proceed genetic monitoring on mice since it is able to distinguish the genetic properties of 9 strains of mouse. |
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| Keywords: | RAPD genetic monitoring |
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