| RNAi表达载体对宫颈癌HPV16 E6基因表达的抑制作用 |
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| 引用本文： | 李大可,彭芝兰,牛小宇,王丹青.RNAi表达载体对宫颈癌HPV16 E6基因表达的抑制作用[J].四川大学学报(医学版),2005,36(3):331-333. |
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| 作者姓名： | 李大可 彭芝兰 牛小宇 王丹青 |
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| 作者单位： | 四川大学华西第二医院,妇产科,成都,610041 |
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| 基金项目： | 国家自然科学基金 (批准号 3 0 3 714 83 )资助 |
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| 摘 要： | 目的研究RNA干扰(RNA interference，RNAi)表达载体对宫颈癌HPV16E6基因的抑制作用。方法构建针对HPV16E6基因的RNAi质粒表达载体，脂质体法转染宫颈癌caski细胞株，应用荧光定量PCR及流式细胞术检测其对HPV16E6mRNA及蛋白表达的影响。结果构建的三种表达载体均抑制了HPV16E6的mRNA及蛋白的表达，其中转染B号载体的caskiB细胞HPV16E6mRNA表达仅相当于空白组的21．7％，蛋白抑制率达98．1％。结论RNAi表达载体可以有效的抑制HPV16E6基因的表达。
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| 关 键 词： | RNA干扰人乳头状瘤样病毒 表达载体FQ—PCR |
| 修稿时间： | 2004年10月8日 |
The Inhibitory Effects of siRNA Expression Vector on the Expression of Human Papillomavirus E6 Gene |
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| LI Da-ke,PENG Zhi-lan,NIU Xiao-yu,WANG Dan-qing.The Inhibitory Effects of siRNA Expression Vector on the Expression of Human Papillomavirus E6 Gene[J].Journal of West China University of Medical Sciences,2005,36(3):331-333. |
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| Authors: | LI Da-ke PENG Zhi-lan NIU Xiao-yu WANG Dan-qing |
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| Institution: | Department of Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China. |
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| Abstract: | Objective To investigate the inhibitory effects of RNAi(RNA interference)expression vector on the expression of human papilomavirus E6 gene. Methods siRNA expression vectors were constructed to be aimed directly at HPV16 E6 gene. The recombinants were transfected into cervical cancer cell line, caski, with liposomes. Expression of E6 was detected with fluorescence quantitative PCR(FQ-PCR) and flow cytometry. Results Three kinds of expression vectors could reduce the expression of E6 mRNA and protein all in caski-B cell. The expression of E6 mRNA reduced to 21.7% of control group, and the protein inhibition ratio reached 98 1%. Conclusion The RNAi expression vector can effectively inhibit the expression of HPVE6 gene. |
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| Keywords: | RNA interference(RNAi) Human papillomavirus Expression vector Fluorescence quantitative PCR(FQ-PCR) |
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