黄药子乙醇提取物抗炎活性研究

目的：观察黄药子乙醇提取物（ET）的抗炎活性。方法：①采用小鼠二甲苯致耳肿胀炎症模型，考察ET（50、100、200mg／kg）的抗炎活性，并考察了在二甲苯诱导的小鼠炎症病理状态下ET对肝功能的影响；②通过分析比较耳内前列腺素PGE2含量，初步探讨了其抗炎机制。结果：①与模型组比较，ET（100、200mg／kg）组小鼠耳肿胀明显降低（P〈0．05），ET（50mg／kg）有降低趋势（P〉0．05），ET（50、100、200mg／kg）呈现出良好的量效关系；与阳性药阿司匹林（Aspirin，200mg／kg）比较，ET（200mg／kg）组小鼠耳肿胀无明显差异（P〉0．05）；ET（200mg／kg）对二甲苯诱导的炎症病理状态下小鼠血清ALT和AST活力均无影响（P〉0．05），而Aspirin（200mg／kg）则可引起AI』T和AST明显升高（P〈0．05）；②ET（200mg／kg）可明显降低小鼠炎症耳组织内过高的炎症因子PGE：含量（P〈0．05）。结论：ET在100、200mg／kg对二甲苯诱发的小鼠急性炎症模型有效，并在同一剂量（200mg／kg）下与Aspirin抗炎作用相当，且避免了Aspirin用药诱发肝毒性的不良反应；ET抗炎作用机制可能与抑制炎症组织内炎症因子PGE，合成有关。

Study on the Anti - inflammatory Activity of Ethanol Extract of Dioscorea Bulbifera L. Rhizome

Objective ： To investigate the anti - inflammatory activity of the ethanol extract of Dioscorea bulbifera L. rhizome （ET）. Methods ： ①Ear edema induced by dimethylbenzene in mice was applied for evaluating the anti - inflammatory effect of ET, and then serum biomarkers including alanine transaminase （ALT） and aspartate transanimase （AST） for liver injury were also assayed in such mice. ②The anti - inflammatory mechanism was explored by measuring the content of PGE2 in mice edema ear. Results ： （~）Compared with Control, ET （ 100,200 mg/kg） significantly reduced the swelling degree of tumefaction in mice with auricle induced by dimethyl- benzene with no conspicuous difference in serum biomarkers for liver injury. Compared with the positive drug aspirin （200 mg/kg）, there is no significant difference in such swelling degree of ET （200 mg/kg）.②ET significantly reduced the content of PGE2 in mice edema ear. Conclusion ： ET has anti - inflammatory effect at doses of 100 and 200 mg/kg and the mechanism may be associated with reduced content of PGE2 in mice edema ear. Moreover, ET has not only an equivalent anti - inflammatory effect with aspirin at the same dose of 200 mg/kg, but also is avoid of the hepatotoxicity induced by aspirin.