胃癌中E-cadherin基因5'端CpG岛甲基化研究

目的:检测胃癌组织中E-cadherin基因5'端CpG岛启动子区域的甲基化状况,探讨该基因甲基化与胃癌组织学类型、淋巴结转移、年龄、性别之间的关系,研究该基因甲基化在胃癌发生发展过程中的作用.方法:采用甲基化特异性PCR方法对胃黏膜中E-cadherin基因5'端CpG岛启动子区域甲基化状况进行研究.结果:胃癌中E-cadherin基因甲基化率为34.6%(28/81),而正常胃黏膜中均未检测到该基因异常甲基化.E-cadherin基因甲基化与肿瘤组织学类型、淋巴结转移及性别均无相关性,但与年龄有关,随着年龄增加患者甲基化率呈增高趋势.结论:E-cadherin基因5'端CpG岛启动子区域甲基化与年龄有关.E-cadherin基因5'端CpG岛启动子区域异常甲基化是胃癌细胞常见的分子事件,可能参与了胃癌的发生发展过程.

Methylation of Gene E-cadherin 5''''CpG Island in Gastric Carcinomas

Objective:To investigate the promoter methylation status of gene E-cadherin in gastric carcinoma, and its correlation with clinical and pathological parameters. Methods:A series of 81 gastric carcinomas and 10 cases of normal gastric tissues were examined for the promoter methylation of E-cadherin by methylation-specific PCR method. Results:Methylation of E-cadherin was found in 34.6% (28/81) of gastric carcinomas, but was not found in the normal gastric mucous membrane.Promoter methylation of E-cadherin was not associated with histological type of the tumor,lymph node metastasis and gender but correlated with age.There is a tendency of increase in methylation with the increase in age.Conclusion:Methylation of E-cadherin is related to age,which is a common molecule event in gastric carcinomas and may play a role in the development and progression of gastric carcinomas.