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Determination of fluoride in human whole blood and urine by gas chromatography-mass spectrometry |
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| Authors: | Shigetoshi Kage Keiko Kudo Naoki Nishida Hideaki Ikeda Naofumi Yoshioka Noriaki Ikeda |
| Affiliation: | (1) Forensic Science Laboratory, Fukuoka Prefectural Police Headquarters, Fukuoka, Japan;(2) Department of Forensic Pathology and Sciences, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan;(3) Department of Legal Medicine, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan;(4) Division of Forensic Sciences, Department of Social Medicine, Akita University School of Medicine, Akita, Japan |
| Abstract: | We developed a simple and sensitive method for determination of fluoride in human whole blood and urine using gas chromatography-mass spectrometry (GC-MS). Fluoride was alkylated with pentafluorobenzyl bromide in a mixture of acetone and phosphate buffer (pH 6.8). The derivative obtained was analyzed by GC-MS in the positive-ion electron-impact mode. The lower limit of detection for the compound was 0.5 mg/l for both matrices. The calibration curve for fluoride was linear over the concentration range of 1–100 mg/l. The precision and accuracy of the method were evaluated, and relative standard deviation was within 10%. Using this method, levels of fluoride in whole blood and urine were determined in a case of poisoning caused by hydrofluoric acid exposure. |
| Keywords: | Fluoride Hydrofluoric acid Gas poisoning GC-MS Pentafluorobenzyl Whole blood |
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