阻塞性睡眠呼吸暂停低通气综合征患者载脂蛋白E基因多态性分析

目的 探讨载脂蛋白E(apoliporotein E,ApoE)基因多态性与阻塞性睡眠呼吸暂停低通气综合征(obstructive sleep apnea hyopnea syndrome,OSAHS)之间的关系.方法 选取经多导睡眠仪确诊的84例无亲缘关系的OSAHS患者(OSAHS组)和排除OSAHS的106位正常人群(正常对照组),采用聚合酶链反应限制性片段长度多态性方法,检测两组ApoE基因多态性的基因型,同时检测两组血清甘油三脂(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇(LDL-C)水平和体质量指数,分析基因型分布、等位基因频率差异、不同等位基因与OSAHS患者血脂水平的关系.结果 OSAHS组ApoEε4等位基因频率明显高于正常对照组,差异有统计学意义(P＜0.01);携带ApoEε4等位基因的OSAHS组患者睡眠呼吸暂停指数高于携带ApoEε3等位基因的OSAHS组患者,差异有统计学意义(P＜0.01);携带ApoEε4等位基因的OSAHS组患者TC水平高于携带ApoEε2和ApoEε3组,差异有统计学意义(P＜0.01).OSAHS组患者TG、LDL-C高于正常对照组,差异有统计学意义(P＜0.05).结论 OSAHS发病与ApoE基因多态性相关联,ApoEε4等位基因町能是OSAHS发病的危险因素.OSAHS患者存在血脂代谢紊乱,携带ApoEε4等位基因的OSAHS患者血清胆固醇升高更明显.

Relationship between apolipoprotein E gene polymorphism and obstructive sleep apnea hypopnea syndrome

Objective To investigate the relationship between apoliprotein E gene polymorphism and obstructive sleep apnea hypopnea syndrome(OSAHS).Methods Polymerase chain reaction-restriction fragment length polymorphisms was performed to determine the polymorphism of apoliprotein E gene in 84unrelated OSAHS subjects(OSAHS group)and 106 non-OSAHS subjects(healthy control group).Body mass index(BMI),Polysomnogram,the levels of total cholesterol(TC),triglyceride(TG),high-density lipoprotein-cholesterol(HDL-C)and low-density lipoprotein-cholesterol(LDL-C)were also analyzed.Results The frequency of ApoEε4 allele in OSAHS group was higher than that in(healthy control group)(P<0.01),AHI in OSAHS patients with ApoEε4 allele was higher than that in OSAHS patients with ApoEε3 allele(P<0.01).The level of TC in OSAHS patients with ApoEε4 allele was higher than that in OSAHS patients with ApoEε3 and ApoEε2 allele(P<0.01).The levels of TG and LDL-C in OSAHS group were significantly higher than those in healthy control group(P<0.05).Conclusions Obstructive sleep apnea hypopnea syndrome might be relative with apoliprotein E gene polymorphism.OSAHS patients have disorder in blood lipids,and OSAHS patients with the allele ε4 of ApoE have higher serum cholesterol.