骨髓间充质干细胞与支架材料nHA/PA66复合培养的生物活性研究 |
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引用本文: | 戚孟春,邓久鹏,董伟,于静,李吉东. 骨髓间充质干细胞与支架材料nHA/PA66复合培养的生物活性研究[J]. 口腔医学研究, 2010, 26(6): 805-808 |
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作者姓名: | 戚孟春 邓久鹏 董伟 于静 李吉东 |
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作者单位: | [1]河北联合大学口腔系,河北唐山063000 [2]四川大学纳米生物材料研究中心,河北唐山063000 |
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基金项目: | 河北省科学技术研究与发展计划项目 |
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摘 要: | 目的:研究骨髓间充质干细胞与支架材料-纳米羟基磷灰石/聚酰胺66(nHA/PA66)复合培养时生物学特性。方法:体外培养兔骨髓间充质干细胞(MSCs),并分为A、B、C、D四组。A组为对照;B组和D组进行骨向诱导;同时,C组和D组细胞与nHA/PA66复合培养。应用MTT实验、碱性磷酸酶(ALP)染色和生物化学分析、扫描电镜,分别对各组细胞的增殖速度、ALP活性及细胞在支架上的黏附情况进行检测。结果:MTT结果显示,A组细胞增殖曲线与C组相似,而B组和D组相似;A组和C组细胞增殖略高于B组和D组。结果表明,骨向诱导可使细胞增殖轻微下降,而与支架材料复合培养对MSCs增殖无影响。ALP染色及ALP活性生化分析进一步表明,MSCs与支架材料复合培养对其骨向分化能力无影响。SEM观察则表明,C组细胞和D组细胞与支架材料均粘附良好。结论:nHA/PA66适于MSCs的黏附、增殖及骨向分化,是一种极具价值的骨组织工程支架材料。
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关 键 词: | 间充质干细胞 纳米羟基磷灰石/聚酰胺66 细胞增殖 骨支架材料 碱性磷酸酶 |
Biological Characteristics of Bone Marrow Mesenchymal Stem Cells Co-cultured with Nano-hydroxyapatite/Polyamide 66 |
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Affiliation: | QI Meng-chun,DENG Jiu-peng,DONG Wei,et al. Department of Stomatology,North China Coal Medical University,Tangshan 063000 |
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Abstract: | Objective:To study the biological characteristics of bone marrow mesenchymal stem cells(MSCs) co-cultured with nano-hydroxyapatite/polyamide 66(nHA/PA66) . Methods:Rabbit bone marrow MSCs were isolated and cultured in vitro. Cells were divided into four groups:A,B,C and D. Group A served as control,while cells in group B and D underwent osteogenic induction;at the same time,cells in group C and D were cocultured with nHA/PA66. Cell proliferation,alkaline phosphatase(ALP) activity and cell adhension on scaffold,were detected by MTT test,ALP staning and biochemical analysis,and scanning electron microscope(SEM) ,respectively. Results:MTT test showed that cell proliferation in group A and group C were similar,so were in group B and D. However,cell proliferation of group A and C were higher than those of group B and D. This indicated that although osteogenic induction could slightly reduce MSCs proliferation,coculture of MSCs with nHA/PA66 had no effect on cell proliferation. ALP staining and biochemical analysis further demonstrated that the osteogenic differentiation of MSCs was not affected by co-culture of MSCs with bone scaffold. SEM observation verified that good cell adhesion was obtained in both C and D. Conclusion:nHA/PA66 was suitble for MSCs adhension,proliferation and osteogenic differentiation,and was a valuable scaffold for bone tissue engineering. |
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Keywords: | Mesenchymal stem cells Nano-hydroxyapatite/polyamide 66 Cell proliferation Bone scaffold Alkaline phosphatase |
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