吸烟诱导慢性阻塞性肺疾病急性加重期血清锁链素水平变化及其与肺功能的相关性研究

目的 探讨吸烟对血清中锁链素(desmosine,DES)表达水平的影响及其与慢性阻塞性肺疾病(COPD)的关系.方法 将COPD急性加重患者分为不吸烟COPD组、吸烟COPD组,同时选择吸烟健康组和不吸烟健康组.收集静脉血,ELISA方法测定血清中的锁链素浓度,对锁链素与吸烟指数、肺通气功能指标FEV1％pred进行直线相关分析.结果 1.吸烟COPD组血清锁链素水平(0.38±0.17) ng/ml高于不吸烟COPD组(0.29±0.08) ng/ml、吸烟健康组(0.21±0.16) ng/ml、不吸烟健康组(0.15±0.07)ng/ml,差异有统计学意义(P＜0.05).2.血清锁链素水平与吸烟指数呈正相关(r=0.412,P＜0.05),与FEV 1％pred呈负相关(r=-0.348,P＜0.05).结论 吸烟COPD患者、不吸烟COPD患者均有使锁链素水平升高的趋势,在吸烟COPD患者中升高更明显.吸烟COPD患者血清锁链素水平与肺功能FEV1％pred下降相关.

Study of changes in the levels of serum desmosine in smoking-induced acute exacerbation chronic obstructive pulmonary disease and its correlation with lung function

Objective To investigate the impact of smoking on the expression of serum desmosine (DES) and its relationship with chronic obstructive pulmonary disease (COPD).Methods Patients with AECOPD were divided into non-smoking COPD group and smoking COPD group,healthy smokers and healthy non-smokers were selected.After collecting venous blood,the serum concentration of desmosine was measured by enzymelinked immunosorbent assay (ELISA),then the linear correlation analysis between desmosine and smoking index,pulmonary function indicator (FEV1％pred) was carried out.Results 1.The serum concentration of desmosine in smoking COPD group (0.38±0.17)ng/ml] was significantly higher than that in non-smoking COPD group,smoking healthy group,and non-smoking healthy group (0.29±0.08)ng/ml,(0.2 1±0.1 6)ng/ml,(0.15±0.07)ng/ml],with statistically significant differences (P＜0.05).2.The serum concentration of desmosine was positively correlated with smoking index (r=0.412,P＜0.05),and negatively correlated with FEV1％pred (r=-0.348,P＜0.05).Conclusion Smokers with COPD and non-smokers with COPD both had higher concentration of desmosine,especially the former.The serum concentration of desmosine was correlated with lung function indicator (FEV1％pred) in smokers with COPD.