荧光PCR和PFGE在副溶血弧菌食物中毒诊断中的应用研究

目的]探讨荧光PCR和脉冲场电泳（PFGE）在副溶血弧菌食物中毒调查中的应用。方法]对中毒样品进行荧光PCR检测,副溶血弧菌临床分离株基因组DNA经NotI酶切,通过PFGE获得电泳图谱,利用BioNumerics软件对图谱进行聚类分析。结果]部分样品荧光PCR检测结果呈阳性,同起食物中毒菌株PFGE图谱一致。结论]荧光PCR可以快速准确的鉴定病原体。PFGE具有很强的菌株同源性分析能力,适用于食物中毒病原菌学鉴定和传染源溯源。荧光PCR和PFGE在食物中毒的快速诊断中可发挥重要作用。

APPLICATION OF REAL-TIME PCR AND PULSED-FIELD GEL ELECTROPHORESIS IN THE DIAGNOSIS OF VIBRIO PARAHAEMOLYTICUS FOOD POISONING

Objective]To explore the application of real-time PCR and pulsed-field gel electrophoresis(PFGE)in the investigation of Vibrio parahaemolyticus food poisoning.Methods]The poisoning samples were assayed by real-time PCR. Vibrio parahaemolyticus strains were analyzed by pulsed-field gel electrophoresis with restriction enzyme NotI. PFGE patterns of isolates were clustered using BioNumerics software.Results]Some samples were positive using real-time PCR assay. PFGE patterns of strains from the same outbreak of food poisoning were identical.Conclusions]Real-time PCR can be applied in the rapid detection of Vibrio parahaemolyticus. PFGE has high resolution,which enables itself to identify pathogen and trace infection sources. Real-time PCR and PFGE should play important roles in food poisoning diagnosis.