Wnt/β-catenin通路及靶基因c-myc在肝细胞癌组织中的表达

目的 观察Wnt通路激活在肝细胞癌起源中的作用.方法 应用免疫组织化学方法检测31例肝细胞癌患者肿瘤组织(实验组)与癌旁正常组织(对照组)中Wnt2、β-catenin及其靶基因c-myc的表达.结果 在肿瘤组织与癌旁正常组织中,Wnt2蛋白的表达差异无统计学意义(P＞0.05);β-catenin的胞质表达在癌旁组织中明显增高(P＜0.05),而其胞核表达则在肿瘤组织中增高(P＜0.05);c-myc在肿瘤组织中表达增高,两组差异有统计学意义(P＜0.01).结论 β-catenin及其靶基因c-myc在肝细胞癌组织中表达增高,提示Wnt通路激活的过程在肝细胞癌的起源中起到重要作用.

Abstract：

Objective To explore the effect of Wnt signaling pathway activation in the origination of hepatocellular carcinoma. Methods Immunohistochemistry was used to observe the expression of Wnt2,β-catenin and target gene c-myc in hepatocellular carcinoma (experimental group) and peficancerous tissue (control group) from 31 patients. Results The expression of Wnt2 had no significantly difference in both groups (P ＞0. 05). The expression of β-catenin in cytoplasm was significantly higher in peficancerous tissue. However,the expression of β-catenin in nucleus was significantly higher in hepatocellular carcinoma ( P ＜ 0. 05). The expression of c-myc was also significantly higher in hepatocellular carcinoma (P ＜ 0. 01).Conclusion β-catenin and it' s target gene c-myc overexpressed in hepatocellular carcinoma. The activation of Wnt signaling pathway played an important role in the origination of hepatocellular carcinoma.