骨骼肌线粒体通透转换孔在骨质疏松症中的变化

目的 观察骨骼肌线粒体通透转换孔(MPTP)的变化,探讨骨骼肌与骨质疏松症的关系.方法 选择因腰椎间盘突出或者腰椎管狭窄症行手术治疗的女性骨质疏松患者13例,另选同时期需上述手术治疗的女性非骨质疏松患者13例.于术中收集肌肉组织,提取骨骼肌线粒体,进行细胞色素C氧化酶(COX)和MPTP的活性检测,并进行相关分析.结果 在MPTP活性变化的比较方面,两组差异有统计学意义(P<0.05).在线粒体COX的活性比较方面,两组差异有统计学意义(P<0.05).在MPTP与COX的相关分析中,MPTP的A比值与COX的活性呈正相关 (r=0.405,P<0.05).结论 骨质疏松骨骼肌的MPTP的通透性高于非骨质疏松,而COX的活性对MPTP的通透性有重要影响,揭示只要维持COX的活性,就能有效控制MPTP的变化,从而有效防治细胞的过早衰老.

Abstract：

Objective To investigate the relationship between osteoporosis and skeletal muscle by studying the change mechanism of the mitochondrial permeability transition pore (MPTP) of skeletal muscle. Methods Twenty-six female patients were selected between May 2008 and December 2008, who were diagnosed as having lumbar intervertebral disc herniation or lumbar spinal stenosis receiving surgical operation. They were divided into osteoporosis group (n=13) and non-osteoporosis group (n=13). Mitochondrions were extracted from the muscle tissue that were collected during the operation.Cytochrome Coxidase (COX) and MPTP were detected. Results The absorbance (A) ratio of MPTP in non-osteoporosis and osteoporosis groups was 0.994±0.007 and 0.985±0.006 respectively (P<0.05). The COX activity in non-osteoporosis and osteoporosis groups was 0.338±0.142 and 0.124±0.093 respectively (P<0.05). The correlation analysis revealed that there was a positive correlation between the A ratio of MPTP and COX activity (r=0.405,P<0.05). Conclusion The permeability of MMPT in osteoporotic muscle is greater than non-osteoporosis group, and COX is important for the permeability of MPTP, indicating that as long as maintaining the activity of COX, the change of MPTP could be effectively controlled, and cell aging would be prevented.

Changes of mitochondrial permeability transition pore of skeletal muscle in osteoporosis

Objective To investigate the relationship between osteoporosis and skeletal muscle by studying the change mechanism of the mitochondrial permeability transition pore (MPTP) of skeletal muscle. Methods Twenty-six female patients were selected between May 2008 and December 2008, who were diagnosed as having lumbar intervertebral disc herniation or lumbar spinal stenosis receiving surgical operation. They were divided into osteoporosis group (n=13) and non-osteoporosis group (n=13). Mitochondrions were extracted from the muscle tissue that were collected during the operation.Cytochrome Coxidase (COX) and MPTP were detected. Results The absorbance (A) ratio of MPTP in non-osteoporosis and osteoporosis groups was 0.994±0.007 and 0.985±0.006 respectively (P<0.05). The COX activity in non-osteoporosis and osteoporosis groups was 0.338±0.142 and 0.124±0.093 respectively (P<0.05). The correlation analysis revealed that there was a positive correlation between the A ratio of MPTP and COX activity (r=0.405,P<0.05). Conclusion The permeability of MMPT in osteoporotic muscle is greater than non-osteoporosis group, and COX is important for the permeability of MPTP, indicating that as long as maintaining the activity of COX, the change of MPTP could be effectively controlled, and cell aging would be prevented.