应力环境对组织培养法保存的人骨软骨移植物的影响

目的 研究应力环境对普通组织培养基体外保存的骨软骨移植物的保存质量的影响,从而改进组织培养法保存软骨的效果.方法用摇床作为动态加载装置对体外保存的骨软骨移植物进行持续周期性应力加载,以模拟体内关节软骨的应力环境.摇床在37℃的孵箱中工作,频率设置为1 Hz.在储存2周时,用Western bloting的方法对关节软骨中的肌动蛋白β-actin进行半定量分析,用透射电镜观察胶原的超微结构,并与对照组比较.结果与静态环境下保存相比,动态加载环境使关节软骨细胞外基质中β-actin的表达水平明显提高,并能更好地保护基质中胶原成分的超微结构.结论应力环境有利于保存人同种异体移植物软骨细胞和胶原的超微结构.动态力学加载可提供一种更好的库存人同种异体骨软骨移植物的方法.

Abstract：

Objective To study the effect of the loading environment on the chondrocytes and the collagen ultrastructure at articular cartilage of the osteochondral allograft graft preserved by tissue culture method so as to improve the preservation effect. Methods A kind of shaking table was used as a dynamic loading device for storage of the osteochondral allograft to simulate the mechanical environment of normal articular cartilage in vivo. The device worked in an incubator at 3711 and the frequency was 1Hz. Western blotting was used for analysis of β-actin at the articular cartilage of the graft for investigating the effects of stress condition on the chondrocyte function. Transmission electron microscopy was used to observe the collagen ultrastructure at the articular cartilage of the graft two weeks after storage and compared with the control group. Results The dynamic loading condition could significantly increase the expression of β-actin and keep the ultrastructure of the collagen fibril in articular cartilage compared with the static condition. Conclusions The loading condition benefits the chondrocyte function and collagen ultrastructure of the articular cartilage in human osteochondral allograft. The dynamic mechanical loading may provide a better method in preservation of the human osteochondral allograft.

Effect of loading environment on the human osteochondral allograft preserved by tissue culture

Objective To study the effect of the loading environment on the chondrocytes and the collagen ultrastructure at articular cartilage of the osteochondral allograft graft preserved by tissue culture method so as to improve the preservation effect. Methods A kind of shaking table was used as a dynamic loading device for storage of the osteochondral allograft to simulate the mechanical environment of normal articular cartilage in vivo. The device worked in an incubator at 3711 and the frequency was 1Hz. Western blotting was used for analysis of β-actin at the articular cartilage of the graft for investigating the effects of stress condition on the chondrocyte function. Transmission electron microscopy was used to observe the collagen ultrastructure at the articular cartilage of the graft two weeks after storage and compared with the control group. Results The dynamic loading condition could significantly increase the expression of β-actin and keep the ultrastructure of the collagen fibril in articular cartilage compared with the static condition. Conclusions The loading condition benefits the chondrocyte function and collagen ultrastructure of the articular cartilage in human osteochondral allograft. The dynamic mechanical loading may provide a better method in preservation of the human osteochondral allograft.