| 一氧化氮对正常及紫外线照射后人表皮细胞凋亡的影响 |
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| 引用本文： | 谭雪晶,刘晓明.一氧化氮对正常及紫外线照射后人表皮细胞凋亡的影响[J].中国皮肤性病学杂志,2011,25(6). |
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| 作者姓名： | 谭雪晶 刘晓明 |
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| 作者单位： | 大连医科大学附属第一医院皮肤科,辽宁,大连,116011 |
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| 摘 要： | 目的探讨一氧化氮(NO)对正常及紫外线照射后人表皮细胞凋亡的影响。方法于健康人皮肤外用两种NO供体,通过真皮血流变化曲线确定与5%亚硝酸盐产生相近NO生理效应的沸石一氧化氮霜的浓度,并通过化学发光法比较二者真皮浅层NO含量。测定各志愿者311nm UVB照射后最小红斑量(MED),以2倍MED的UVB照射皮肤,于照射及非照射部位外用沸石一氧化氮霜及空白对照剂,应用组织切片HE染色和Caspase3,p53免疫组化染色检测表皮细胞凋亡,比较各组凋亡细胞数。结果①33%沸石一氧化氮霜与5%亚硝酸盐霜外用于皮肤后,二者血流曲线相近,真皮浅层NO水平二者比较差异无统计学意义(P>0.05);②2倍MED紫外线照射后表皮细胞凋亡明显增加(P<0.01),外用NO供体诱导表皮细胞凋亡不明显(P>0.05);③紫外线照射后24h,外用NO供体处凋亡的表皮细胞较外用对照剂处明显减少(P<0.05)。结论 NO对正常人表皮细胞无明显致凋亡作用,对UVB照射诱发的表皮细胞凋亡有明显抑制作用。
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| 关 键 词： | 一氧化氮 表皮细胞 凋亡 UVB |
The Effects of Nitric Oxide on Apoptosis in Normal and UVB Irradiated Human Epidermal Cells |
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| TAN Xue-jing,LIU Xiao-ming.The Effects of Nitric Oxide on Apoptosis in Normal and UVB Irradiated Human Epidermal Cells[J].The Chinese Journal of Dermatovenereology,2011,25(6). |
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| Authors: | TAN Xue-jing LIU Xiao-ming |
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| Institution: | TAN Xue-jing,LIU Xiao-ming(Department of Dermatology,the First Affiliated Hospital of Dalian Medical University,Dalian 116011,China) |
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| Abstract: | Objective To explore the effects of nitric oxide(NO) on apoptosis in normal and ultraviolet B(UVB) treated human epidermal cells in vivo.Methods Determine the concentration of Zeolite-NO cream which produce similar biophysical effect with 5% acidified nitrite by means of blood flow measurement and compare the concentration of NO in upper dermis by chemiluminescence analysis following application of two kinds of NO donors on healthy human skin.Determine the MED value of each healthy volunteer after 311nm UVB... |
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| Keywords: | UVB |
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