神经生长因子缓释微球移植对AD模型鼠基底前脑ChAT阳性神经元的保护作用

目的观察神经生长因子微球对AD模型鼠基底前脑ChAT阳性神经元的保护作用。方法采用双乳化技术制备神经生长因子缓释微球;切断SD大鼠左侧穹隆海马伞,基底前脑注射神经生长因子缓释微球;4周后,利用免疫组化法观察各组大鼠基底前脑ChAT阳性神经元变化。结果损伤组损伤侧的MS和VDB的ChAT阳性神经元大量减少,分别减少61.9%和51.4%;神经生长因子缓释微球治疗组损伤侧的ChAT阳性神经元得到明显的保护,MS和VDB细胞数分别下降20.60%和20.9%,明显高于损伤组损伤侧的ChAT阳性神经元存活数。结论神经生长因子缓释微球能够成功地将神经生长因子运载到脑内,神经生长因子缓释微球移植对AD模型鼠基底前脑ChAT阳性神经元有明显的保护作用。

Effect of implantation of rhngf microspheres on the chat-positive neurons of the basal forebrain after fornix-fimbria transections

Objective To study the effect of implantation of rhNGF microspheres on the ChAT-positive neurons of the basal forebrain after fornix-fimbria transactions. Methods Recombinant human NGF microspheres were prepared by a W/O/W emulsion and solvent evaporation technique with some modifications. Recombinant human NGF microspheres were transplanted into the basal forebrain after fornix-fimbria transactions. After 4 weeks, ChAT-positive neurons of the basal forebrain in the different groups were observed by immunohistochemistry. Results After 4 weeks, in LES group, the percentages of ChAT-positive neurons at the lesion side to the intact side of MS and VDB were 38.1% and 48.6%, respectively. In MIC group, the percentages of ChAT-positive neurons at the lesion side to the intact side of MS and VDB were 79.4% and 79.1%, respectively, which were significantly more than that in LES group (P<0.01). Conculsions NGF can be successfully deliveried to the brain by recombinant human NGF microspheres. Recombinant human NGF microspheres promote the survival of ChAT-positive neurons of the basal forebrain after fornix-fimbria transections.