| 大孔树脂吸附法精制牡丹皮总苷的研究 |
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| 引用本文： | 刘成红,程斌,曹红,邢俊波,陈玉敏.大孔树脂吸附法精制牡丹皮总苷的研究[J].中国新药杂志,2006,15(10):807-809. |
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| 作者姓名： | 刘成红 程斌 曹红 邢俊波 陈玉敏 |
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| 作者单位： | 总后卫生部药品仪器检验所中药室,北京,100071 |
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| 摘 要： | 目的：筛选大孔树脂吸附法精制丹皮总苷的最佳条件.方法：采用动态吸附-解吸方法,用HPLC法测定芍药苷的含量对工艺进行评价.结果：上柱量按1 mL树脂吸附3.2 mg芍药苷计,以6倍柱体积水洗脱;收集40%乙醇洗脱液,洗脱剂用量约为6倍柱体积.丹皮总苷得率为3.7%,含芍药苷为17%.结论：该法简单可行,分离效果好,能满足大生产的要求.
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| 关 键 词： | 丹皮总苷 大孔树脂 芍药苷 |
| 文章编号： | 1003-3734（2006）10-0807-03 |
| 修稿时间： | 2005-12-27 |
Refinement of total glucosides from mudan cortex by macroporous resin |
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| LIU Cheng-hong,CHENG Bin,CAO Hong,XING Jun-bo,CHEN Yu-min.Refinement of total glucosides from mudan cortex by macroporous resin[J].Chinese Journal of New Drugs,2006,15(10):807-809. |
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| Authors: | LIU Cheng-hong CHENG Bin CAO Hong XING Jun-bo CHEN Yu-min |
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| Institution: | Institute of Quality Control of Drugs and Instruments, Department of Health Care, General Logistics Department of PLA, Beijing 100071, China |
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| Abstract: | Objective:To develop an optimal condition to separate total glucosides cortex from moutan cortex by macroporous resins.Methods:The total glucosides were separated over a dynamic ab- sorption-desorption process.The separation efficiency was evaluated by measuring concentration of paeon- iflorin by HPLC.Results:The optimal separation conditions were as followed:loading column ratio of the resins to the glucosides (based on paeoniflorin weight) in 1 mL:3.3 mg;an eluate consisted of 6 times of water (resin bed volume) and 40% ethanol,and then 40% ethanol eluation.The yield of total glueosides was 3.7%,and paeoniflorin 17%.Conclusion:This separation method is warranted for the pilot process. |
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| Keywords: | total glucosides from mudan cortex macroporous resin paeoniflorin |
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