miR-203在系统性红斑狼疮患者外周血单个核细胞中的表达及临床意义

目的:探讨系统性红斑狼疮(systemic lupus erythematosus,SLE)患者外周血单个核细胞中miR-203的表达水平与疾病活动度?肾脏受累情况等临床表现之间的关系,初步探讨miR-203在SLE中的临床意义?方法:收集31例SLE患者?16例健康志愿者外周血标本,根据肾脏受累分为狼疮肾炎21例和SLE无肾脏受累10例?采用实时荧光定量聚合酶链反应(real-time fluorescence quota polymerase chain reaction,qRT-PCR)检测外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)中miR-203表达,比较各组间miR-203表达水平的差异,并分析其与SLE临床指标之间的关系?结果:SLE患者miR-203表达明显低于正常对照组,差异具有统计学意义(P < 0.05);狼疮肾炎组患者miR-203表达低于SLE无肾脏受累组和正常对照组,差异具有统计学意义(P < 0.01);SLE患者miR-203表达水平与24 h尿蛋白?肾脏急性指数呈明显负相关(P < 0.01)?结论:SLE患者PBMCs中miR-203的表达水平降低,表明miR-203在SLE发病机制中可能发挥一定作用;狼疮肾炎患者miR-203的表达降低,提示miR-203可能参与了狼疮肾炎的发病?

Expression of miR-203 in peripheral blood mononuclear cells in patients with systemic lupus erythematosus

Objective:To explore the expression level of miR-203 in the peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus (SLE), and to analyze the relationship between the expression level and clinical manifestations. Methods: The PBMCs of 31 SLE patients, 16 healthy people were collected,and then SLE patients were divided into two groups, including the lupus nephritis group (21 cases) and the no kidney involvement group (10 cases). The expression level of miR-203 in PBMCs was detected by real-time fluorescence quota polymerase chain reaction (qRT-PCR). The expression levels of miR-203 in each group were compared to analyze the relationship between the expression level and clinical manifestations. Results: The expression level of miR-203 was significantly lower in the SLE group compared to the control group (P < 0.05). The expression level of miR-203 was significantly lower in the lupus nephritis group than that in the no kidney involvement group and the control group (both P < 0.01). The expression level of miR-203 was negative correlated to 24 hours urine protein, acute kidney disease index (P < 0.01). Conclusion: The expression of miR-203 was decreased in SLE patients, indicating that miR-203 may play a role in the pathogenesis of SLE. And miR-203 may be associated with the occurrence of lupus nephritis.