二乙基亚硝胺所诱导大鼠肝癌表达上调的基因

目的:首次大范围地观察二乙基亚硝胺(DEN)诱导的大鼠肝癌组织基因表达的差异,探索DEN诱发肝癌的分子机制.方法:DEN诱导大鼠肝癌,常规抽提和纯化RNA,采用AffymetrixRat230AGeneChip及技术比较肝癌组织与正常大鼠肝组织基因表达的差异.结果:在芯片的15710个基因中,肝癌有84.54%的基因阳性表达,肝癌基因表达在正常肝脏5倍以上的有509个,其中325个为EST片段,已知基因184个,其中的168个基因可以检索到有关文献的报道.在这168个基因中,有100个基因被发现与肿瘤有关,其中有36个与肝癌有关;有4个基因与肝脏有关;另有64个基因与肿瘤和肝脏无关.结论:?DEN诱发大鼠肝癌的后基因组变化中168个基因值得优先关注.

Up-regulated genes in diethylnitrosamine-induced liver cancer in rats

AIM: To investigate the differentially expressed genes in rat liver cancer induced by diethylnitrosamine (DEN), and to explore mechanism in DEN-induced carcinogenesis. METHODS: The model of liver cancer was induced by DEN. The RNA was extracted purified from the cancer cells with routine methods. Affymetrix Rat 230A GeneChip and technique were used to detect the dis- crepancies of gene expression between tissues of nor- mal control and liver cancer. RESULTS: Of 15 710 genes in the GeneChip, the positive expression covered a percentage of 84.54% in liver cancer. There were 509 genes whose expression levels in liver cancer were about 5 times as high as those in the normal controls, of which 325 were EST fragments. Of the 325 genes, 184 ones were with known function, of which 168 had been reported in relevant references.Of the 168 genes, 100 ones were associated with the tumor (36 with liver cancer), 4 with liver, and the other 64 had no relation with tumor and liver. CONCLUSION: DEN-induced up-regulations of genes in liver cancer are fairly complicated, of which 168 ones need paying more attention to.