|
|||||
|
|
| 信号标签诱变突变体文库穿梭质粒构建 | |
| 引用本文： | 刘德松,王涛,陈建平,鲁芳,张雷,杨志伟.信号标签诱变突变体文库穿梭质粒构建[J].西部医学,2006,18(1):4-6. |
| 作者姓名： | 刘德松 王涛 陈建平 鲁芳 张雷 杨志伟 |
| 作者单位： | 1. 四川大学华西医学中心形态学实验室、寄生虫学教研室,四川,成都,610041 2. 四川大学华西医学中心生物医学工程研究室,四川,成都,610041 3. 大理医学院微生物学教研室,云南,大理,671000 4. 宁夏医学院微生物学教研室,宁夏,银川,750004 |
| 摘 要： | 目的建立信号标签诱变突变体穿梭质粒,为构建信号标签诱变突变体文库及病原微生物毒力基因筛选打下基础。方法通过PCR扩增信号标签(signature tags,STs)片段,导入载体pC6,构建重组质粒pC6RS,转化到大肠杆菌CC118中,提取重组质粒后转化到大肠杆菌S17-1中。结果经过PCR、核苷酸序列测定鉴定,成功地构建了pC6RS质粒。 |
| 关 键 词： | 信号标签诱变 穿梭质粒 突变体文库 |
| 文章编号： | 1672-3511（2006）01-0004-03 |
| 收稿时间： | 2005-10-18 |
| 修稿时间： | 2005年10月18 |
Shuttle plasmid construction of Signature-Tagged Mutagenesis technology |
|
| LIU De-song,WANG Tao,CHEN Jian-ping,LU Fang,Zhang Lei,YANG Zhi-wei.Shuttle plasmid construction of Signature-Tagged Mutagenesis technology[J].Medical Journal of West China,2006,18(1):4-6. | |
| Authors: | LIU De-song WANG Tao CHEN Jian-ping LU Fang Zhang Lei YANG Zhi-wei |
| Institution: | 1. Department of Parasiotology and Lab of Morphology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041,China; 2. Department of Biomedical Engineering, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041 ,China; 3. Department of Microbiology, Dali Medical College, Dali 671000, China; Department of Microbiology, Nin gxia Medical College, Yinchuan 671000, China |
| Abstract: | Objective To construct the shuttle plasmid of Signature-Tagged Mutagenesis technology and lay the basis to establish the mutant library and virulence genes selection of pathogenic microorganism.Methods The DNA signature tags was obtained by PCR from the single stands DNA tags.The signature tags was cloned into pC6 to construct recombinant plasmid pC6RS.Then it was transformed into E.coli CC118 to produce multiple recombinant plasmid.pC6RS extracted from CC118 was subsequently transformed into E.coli S17-1. Results The recombinant plasmid pC6RS identified by enzyme digestion and DNA sequencing was successfully constructed. |
| Keywords: | Signature-tagged mutagenesis Shuttle plasmid Mutant library |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |