复发性生殖器疱疹患者外周血T淋巴细胞和共刺激分子的检测

目的检测复发性生殖器疱疹(RGH)患者外周血中T细胞亚群和共刺激分子的表达,探讨其在RGH免疫中的作用。方法用免疫荧光技术和流式细胞仪检测RGH及对照组血中T细胞亚群和共刺激分子(CD28,CD25,Fas,FasL,4-1BB)的水平。结果 RGH组CD3+,CD4+/CD8+,CD4+CD28+细胞百分数(59.48±10.9,0.89±0.20,27.69±7.34)显著低于健康对照组(69.79+5.63,1.14+0.39,33.68±7.32),差异均有统计学意义(P<0.05);CD4+CD28-,CD25+细胞百分数及Fas(6.55±4.46,15.81±8.62,27.58±3.10)显著高于健康对照(1.93±1.36,7.13±5.13,23.11±5.63),差异均有统计学意义(P<0.01,P<0.05)。结论 RGH外周血CD3+,CD4+/CD8+下降,CD4+CD28+低表达,及CD25+和Fas高表达,可能是RGH复发的免疫障碍环节之一。

T Cell Subsets and Costimulatory Molecule in Peripheral Blood of Patients with Recurrence Genital Herpes

Objective To detect the expression of T cell subsets and related costimulatory molecule in peripheral blood of patients with recurrence genital herpes (RGH),and explore their contributions in the RGH immunopathogenesis. Methods The expression of some costimulatory molecules (CD28,CD25,Fas,FasL,4-1BB) and T cell subsets in peripheral blood of patients with RGH and healthy controls were detected by immunofluorescence technique and Flow Cytometry. Results The percentage of CD3+,CD4+/CD8+ and CD4+ CD28+cells ratio in patients(59.48±10.9,0.89±0.20,27.69±7.34) were all lower than that in controls(69.79+5.63,1.14+0.39,33.68±7.32)(P<0.05).The percentage of CD4+CD28-cells and the regulatory CD25+ T cells,Fas in patients(6.55±4.46,15.81±8.62,27.58±3.10) was significantly higher than that in the controls(1.93±1.36,7.13±5.13,23.11±5.63)(P<0.01). Conclusion The lower expression of CD4+CD28+ T cells in RGH patients suggests that the T cell activation of RGH patients are impairment. There are higher expression of CD25+ T cells in RGH patients,and its negative regulation plays an important role in inhibiting the Organism's cell immunity reaction.