草乌净制机制探析

目的: 探析草乌的净制机制。 方法: 采用HPLC测定3批带残茎草乌、去残茎草乌及草乌残茎中新乌头碱、乌头碱、次乌头碱、苯甲酰新乌头碱、苯甲酰乌头碱、苯甲酰次乌头碱的含量。 结果: 带残茎草乌、去残茎草乌、草乌残茎中3种双酯型生物碱的总含量分别是0.59%~0.66%,0.61%~0.69%,0.39%~0.42%;3种单酯型生物碱的总含量分别是0.09%~0.10%,0.09%~0.12%,0.06%~0.08%。草乌不同部位的色谱图中除6种酯型生物碱峰外还存在共有峰4,残茎中峰4的峰面积是去残茎草乌的55%~80%。 结论: 草乌残茎和去残茎草乌中6种生物碱成分含量存在差异;残茎属于生物碱成分含量较低的非药用部位,需去除。

Analysis of Cleansing Mechanism of Aconitum kusnezoffii

Objective: To analyze cleansing mechanism of Aconitum kusnezoffii. Method: HPLC was adopted to determine the content of mesaconine,aconitine,hypaconitine,benzoylmesaconine,benzoylaconitine,benzoylhypaconitine in A. kusnezoffii with stems,A. kusnezoffii without stems and stems of A. kusnezoffii. Result: The total content of three kind of diester alkaloids in A. kusnezoffii with stems,A. kusnezoffii without stems and stems of A. kusnezoffii was 0.59%-0.66%,0.61%-0.69%,0.39%-0.42%,respectively;The total content of three kind of monoester alkaloids was 0.09%-0.10%,0.09%-0.12%,0.06%-0.08%,respectively.Except six alkaloids chromatographic peaks,there was another common peak(peak 4),area of peak 4 in stems of A. kusnezoffii was 55%-80% to that in A. kusnezoffii without stems. Conclusion: Content of six alkaloids was found in A. kusnezoffii without stems and stems of A. kusnezoffii was different.Stems of A. kusnezoffii was the non-medicinal parts whose content of six alkaloids was low,it could be removed.