比色法测定龙血竭总黄酮含量

目的:对龙血竭总黄酮的比色测定方法进行研究.方法:利用龙血竭总黄酮可与各种不同的显色试剂作用并产生颜色的特性,比较显色后的吸收曲线变化差异.结果:龙血竭用HCl-Mg粉显色后在475 nm处产生一个大的吸收峰,且此处的干扰较小,在6h内稳定性良好,在0.012 ～0.12 g·L-1呈良好的线性关系(r=0.9993);用其他显色剂显色后吸收峰均无太大变化.结论:总黄酮测定常用的AlCl3,NaNO2-Al(NO3)3-NaOH比色法不适合龙血竭总黄酮的测定,HCl-Mg粉可作比色法测定龙血竭总黄酮的显色剂,但仍需寻找合适的对照品.

Content Determination of Total Flavonoids in Resina Draconis by Colorimetric Method

Objective: To study the colorimetric determination of total flavonoids in Resina Draconis. Method: Comparison on the difference of absorption curve was done using the characteristics that total flavonoids of Resina Draconis can produce color with various chromogenic reagents. Result: Resina Draconis colored by HCl-Mg power produce a large absorption peak at 475 nm where the interference is small. It had good stability within 6 h and showed a good linear relationship in the range of 0.012-0.12 g·L^-1 (r=0.999 3). And its absorption peak was not much change after colored by other chromogenic agent. Conclusion: AlCl₃, NaNO₂-Al(NO₃)₃-NaOH colorimetries usually used for determination of total flavonoids were not suitable for Resina Draconis flavones assay. HCl-Mg powder can be used as chromogenic agent for colorimetric determination of the total flavonoids in Resina draconis. But it still needs to find a suitable reference substance.