幽门螺杆菌表达LeWis B抗原不影响细菌的黏附

目的：研究幽门螺杆菌（Hp)表达LewisB抗原对细菌黏附的可能影响，以及Hp的babA2基因与消化性溃疡的关系，方法，采用体外黏附分子法检测细菌的黏附性，聚合酶反应法（PCR）检测Hp的babA2基因，结果：（1）在47例消化性溃疡Hp株中，18株为babA2基因阳性，而31例非溃疡性消化不良Hp株中，14株为babA2阳性（P＝0．427），（2）在表达LewisB抗原的31株Hp中，24株细菌黏附阳性，而不表达LewisB抗原的47株Hp中，38株呈细菌黏附阳性（P＝0．463），结论：（1）Hp的babA2基因与化性溃疡的发生无关，（2）Hp表达LewisB抗原不影响细胞的黏附，从而支持上皮LewisB是Hp的受体。

No influence of Lewis B expression in Helicobacter pylori on bacterial adhesion property

Objective To investigate the possible influence of Lewis B expression in Helicobacter pylori (H.pylori) on bacterial adhesion property, and determine the relationship between babA 2 gene in H.pylori and peptic ulcer. Methods The bacterial adhesion property in a total of 78 H.pylori strains was performed using adherence assay in vitro, and the frequency of babA 2 gene was determined by polymerase chain reaction (PCR). Results Among 47 H.pylori strains isolated from peptic ulcer patients, 18 (38.3%) were positive for babA 2 gene, while babA 2 was positive in 14 (45.2%) of 31 strains isolated from non ulcer dyspepsia patients ( P =0.427). Among 31 H.pylori strains expressing Lewis B, 24 (77.4%) were positive for adherence assay, compared with 38 (80.9%) of 47 H.pylori strains without expression of Lewis B ( P =0.463). Conclusions (1) There is no association between babA 2 status in H.pylori strain and peptic ulcer in our population; (2) The expression of Lewis B in H.pylori does not interfere with bacterial adhesion property, and thus supports that the gastric epithelium is the receptor for Lewis B of H.pylori.