Polycarboxylated microfillers incorporated into light-curable resin-based dental adhesives evoke remineralization at the mineral-depleted dentin |
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Authors: | Zhejun Wang Ya Shen Markus Haapasalo Jiao Wang |
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Institution: | 1. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) &2. Key Laboratory of Oral Biomedicine Ministry of Education, School &3. Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, PR China;4. Division of Endodontics, Oral Biological &5. Medical Sciences, School of Dentistry, University of British Columbia, Vancouver, Canada;6. Division of Endodontics, Oral Biological &7. Laboratory of Molecular Neural Biology, Institute of Systems Biology, School of Life Sciences, Shanghai University, 99 Shang Da Road, Shanghai 200444, China |
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Abstract: | This study aimed at evaluating the remineralizing properties of three experimental light-curable resin-based dental adhesives containing tailored polycarboxylated microfillers. A co-monomers blend was firstly formulated and then mixed with each of the following microfillers: polycarboxylated bioactive glass (PBAG), polycarboxylated calcium silicates (PCS), and polycarboxylated calcium silicates-doped brushite (PDP). The three experimental and a filler-free control resins were applied onto 10% orthophosphoric acid treated dentin discs and light cured. The specimens were soaked in artificial saliva (AS) for 3, 7, and 14?days. Dentin mineral variation was monitored using attenuated total reflection-Fourier transform infrared (ATR-FTIR) and Raman spectroscopy. Confocal laser scanning microscopy (CLSM) was employed to observe the ultra-morphology/nanoleakage along the resin–dentin interface. The bonding ability and the durability of the resin–dentin bonds were investigated through microtensile bond strength (μTBS) test. ATR-FTIR and Raman showed a significant increase of the mineral matrix area ratio and phosphate peak intensity in specimens treated with the experimental resins within 14?days (p?0.05). No significant increment of minerals was found in untreated specimens or specimens treated using the control filler-free resin (p?>?0.05). Dentin treated using PBAG or PCS exhibited higher level of remineralization than the specimens in PDP group. CLSM showed reduction in nanoleakage, although the remineralization of the hybrid layer induced a significant drop in the μTBS after 3-month storage (p?0.05). The experimental resin-based dental adhesives containing bioactive microfillers remineralize the resin–dentin interfaces when in intimate contact with biological fluids. |
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Keywords: | bonded-dentin interface bioactive glass calcium phosphate calcium silicate dentin remineralization resin adhesive |
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