| RP-HPLC法测定广东海风藤中五味子醇甲的含量 |
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| 引用本文： | 翁文,高勤,李晓光,罗焕敏. RP-HPLC法测定广东海风藤中五味子醇甲的含量[J]. 中药材, 2006, 29(6): 567-569 |
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| 作者姓名： | 翁文 高勤 李晓光 罗焕敏 |
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| 作者单位： | 暨南大学药学院,广东广州510632 |
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| 基金项目： | 广东省科技厅科技计划;广东省中医药管理局科研项目 |
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| 摘 要： | 目的:建立RPHPLC测定广东海风藤中五味子醇甲含量的方法。方法:固定相为KromasilC18反相柱(5μm,250mm×4.6mm),流动相为甲醇水(65∶35),检测波长为250nm。结果:该方法的线性范围为0.08～0.60μg(r=0.9998),平均回收率为100.24%,RSD=2.09%(n=5)。结论:本法简便,准确,灵敏度高,重现性好,可用于广东海风藤中五味子醇甲含量的测定。
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| 关 键 词： | 广东海风藤 五味子醇甲 反相高效液相色谱法 |
| 文章编号： | 1001-4454(2006)06-0567-03 |
| 收稿时间： | 2005-12-15 |
| 修稿时间： | 2005-12-15 |
Deterimination of Schisandrin in Kadsura heteroclita (Roxb.) Craib by RP-HPLC |
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| WENG Wen,GAO Qin,LI Xiao-guang,LUO Huan-min. Deterimination of Schisandrin in Kadsura heteroclita (Roxb.) Craib by RP-HPLC[J]. Journal of Chinese medicinal materials, 2006, 29(6): 567-569 |
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| Authors: | WENG Wen GAO Qin LI Xiao-guang LUO Huan-min |
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| Affiliation: | Jinan University, Guangzhou 510632, China. |
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| Abstract: | OBJECTIVE: To develop a method of RP-HPLC for determination of schisandrin content in Kadsura heteroclita (Roxb.) Craib. METHOD: RP-HPLC analysis was carried out by using Kromasil C18 column (5 microm, 250 mm x 4.6 mm) and methanol-water (65:35) as the mobile, and the detection wavelength was 250 nm. RESULTS: The method was linear in the range of 0.08-0.06 nicrog (r = 0.9998), and the average recovery was 100.24%, with the RSD of 2.09% (n=5). CONCLUSION: The method was simple, accurate, highly sensitive and reproducible. It may be used for the quantitative determination of schisandrin in extract of Kadsura heteroclita (Roxb.) Craib. |
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| Keywords: | Kadsura heteroclita(Roxb.) Craib Schisandrin RP-HPLC |
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