| 日本血吸虫单克隆抗独特型抗体NP30的建株及初步鉴定 |
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| 引用本文： | 管晓虹,仇镇宁,马磊,陶如华,吴宜琴,河清,吴观陵,赵慰先.日本血吸虫单克隆抗独特型抗体NP30的建株及初步鉴定[J].中国寄生虫学与寄生虫病杂志,1991(4). |
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| 作者姓名： | 管晓虹 仇镇宁 马磊 陶如华 吴宜琴 河清 吴观陵 赵慰先 |
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| 作者单位： | 南京医学院医学检测中心，南京医学院寄生虫学教研室 南京市卫生学校
南京市汉中路140号 210029，南京市汉中路140号 210029，南京市汉中路140号 210029，南京市汉中路140号 210029，南京市汉中路140号 210029，南京市汉中路140号 210029 |
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| 基金项目： | 国家“七五”科技攻关重点项目 |
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| 摘 要： | 用感染8条日本血吸虫尾蚴,时间长达一年半的BALB/c小鼠脾细胞与SP2/0骨髓瘤细胞融合,用兔抗日本血吸虫肠相关抗原和兔抗可溶性虫卵抗原免疫血清与细胞培养上清进行ELISA筛选阳性克隆,最终建立了分泌单克隆抗独特型抗体细胞株NP30。作者用一系列试验证明,NP-30是日本血吸虫肠相关抗原的内影象抗独特型抗体,有替代日本血吸虫虫源性抗原用于血清学诊断的可能性。
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| 关 键 词： | 日本血吸虫 单克隆抗体 抗独特型抗体 肠相关抗原 |
THE ESTABLISHMENT AND CHARACTERIZATION OF THE ANTI-IDIOTYPIC MONOCLONAL ANTIBODY-NP3O OF SCHISTOSOMA JAPONICUM |
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| Guan Xiao-Hong,Qiu Zhen-Ning,Ma Lei,Tao Ru-Hua,Wu Yi-Qin,He Qin,Wu Guan-Ling,Zhao Wei-Xian.THE ESTABLISHMENT AND CHARACTERIZATION OF THE ANTI-IDIOTYPIC MONOCLONAL ANTIBODY-NP3O OF SCHISTOSOMA JAPONICUM[J].Chinese Journal of Parasitology and Parasitic Diseases,1991(4). |
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| Authors: | Guan Xiao-Hong Qiu Zhen-Ning Ma Lei Tao Ru-Hua Wu Yi-Qin He Qin Wu Guan-Ling Zhao Wei-Xian |
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| Abstract: | A hybridoma cell line secreting an IgM monoclonal antibody designated NP30 was ob- tained from a fusion of SP2/O and spleen cells of a BALB/c mouse chronically infected with schistosoma japonicum for one and a half year and identified by screening with immunized rabbit sera against gut-associated antigen (GAA) and soluble egg antigen (SEA) of S. japonicum, indicating that the NP30 was an anti-anti-antigen or anti-antibody. NP30 was further determined to be an anti-idiotypic antibody (anti-id) which was serologically and functionally identical to GAA, so that it could be portraied as the internal image of GAA, which might have the potential to be used as an antigenic reagent in immunodiagnostic assays, of schistosomiasis japonica. |
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| Keywords: | Schistosoma japonicum monoclonal antibody anti-idiotypic antibody gut-associated antigen |
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