Stathmin基因表达与肿瘤化疗药物敏感性的关系

目的:探讨8种肿瘤细胞对长春新碱(vincristine, VCR)和泰索帝(docetaxel, DOC)两种化疗药物的敏感性以及该敏感性与肿瘤细胞内Stathmin基因表达之间的关系. 方法:采用MTT法和形态学观察,根据两种药物对细胞的相对抑制率,分析8种肿瘤细胞对两种药物的敏感情况;应用实时荧光定量PCR技术分析比较上述细胞Stathmin基因表达情况. 结果: 8种肿瘤细胞对VCR的敏感程度(相对抑制率)依次为:骨肉瘤细胞9607,58%,9901, 56%;宫颈癌细胞Hela, 37%;肝癌细胞HHCC, 34%;肺癌细胞A549,31%;喉癌细胞Hep-2,17%;胃癌细胞MKN45, 16%;肝癌细胞7721,15%. 对DOC敏感程度依次为: 9607, 48%; Hela,46%;9901,44%;Hep-2, 34%;A549, 31%;MKN45,15%;HHCC, 12%;7721, 3%;Stathmin基因在各肿瘤细胞中具有不同程度的表达,其表达强度与各肿瘤细胞对两种药物的敏感性有关. 结论:肿瘤细胞中Statnmin基因表达水平与其化疗药物敏感程度有关,检测Statnmin基因表达水平对临床化疗药物的选择有一定的指导意义.

Relationship between expression of Stathmin gene in cancer cells and its sensitivity to chemotherapeutic drugs

AIM: To investigate the sensitivity of eight kinds of cancer cells to two kinds of chemotherapeutic drugs: Vincristine and Docetaxel and to study the relationship between the sensitivity and the expression of Stathmin gene in these cells. METHODS: The chemosensitivity of the eight kinds of cancer cells to Vincristine (VCR) and Docetaxel (DOC) was detected by MTT colorimetric assay and morphological observation. Real time PCR technology was used to examine the expression of Stathmin gene in these cells. RESULTS: The inhibition rates of the eight kinds of cancer cells by VCR and DOC were respectively 58% and 48% for human osteosarcoma cells line Sosp-9607 (9607), 56% and 44% for human osteosarcoma cells line Sosp-9901 (9901), 37% and 46% for cervical cancer cells line Hela (Hela), 34% and 12% for human hepatocellular carcinoma cells line HHCC (HHCC), 31% and 31% for lung cancer cells line A549 (A549), 17% and 34% for largngo-carcinoma cells line Hep-2 (Hep-2), 16% and 15% for gastric cancer cells line MKN45 (MKN45), and 15% and 3% for human hepatocelluar carcinoma cells line 7721 (7721) cells. The intensity of the expression of Stathmin gene in the eight kinds of cells was different and was correlated with the sensitivity mentioned above. CONCLUSION: The expression of Stathmin gene in cancer cells is related to its sensitivity to the chemotherapeutic drugs. The detection of the expression of Stathmin gene in tumor cells may be of some clinical significance in choosing a chemotherapy agent.