| HPLC法测定银杏叶软胶囊中总黄酮的含量 |
| |
| 引用本文： | 叶秀波,岑帼英,李瑞明. HPLC法测定银杏叶软胶囊中总黄酮的含量[J]. 中国医药导报, 2010, 7(33): 29-30,41 |
| |
| 作者姓名： | 叶秀波 岑帼英 李瑞明 |
| |
| 作者单位： | [1]广州市海珠区中医院．广东广州510220 [2]中山大学附属第一医院药学部,广东广州510080 |
| |
| 摘 要： | 目的：采用RP-HPLC法测定保健食品银杏叶软胶囊中总黄酮的含量。方法：采用Agilent C18（4.6 mm×250 mm,5μm）色谱柱,流动相为甲醇-水（用磷酸调pH为2.5）（50∶50）,流速为1.0 ml/min,检测波长为360 nm,柱温为30℃。结果：槲皮素、山奈素、异鼠李素的线性范围分别为0.050～0.400、0.005～0.040、0.005～0.040μg;3批样品中总黄酮含量分别为6.466、6.455、6.838 mg/粒。结论：本法简便、准确、分离效果好,适合于银杏叶软胶囊总黄酮的含量测定。
|
| 关 键 词： | 银杏叶软胶囊 总黄酮 槲皮素 山奈素 异鼠李素 含量测定 |
Determination of Total Flavonoid in Yinxingye Soft Capsules by HPLC |
| |
| YE Xiubo,CEN Guoying,LI Ruiming. Determination of Total Flavonoid in Yinxingye Soft Capsules by HPLC[J]. China Medical Herald, 2010, 7(33): 29-30,41 |
| |
| Authors: | YE Xiubo CEN Guoying LI Ruiming |
| |
| Affiliation: | 1.Traditional Chinese Medicine Hospital of Haizhu District,Guangzhou 510220,China;2.Department of Pharmacy,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China) |
| |
| Abstract: | Objective：To establish an RP-HPLC method for quantitative determination of Total Flavonoid in Yinxingye Soft Capsules.Methods：The analytical column was Agilent C18（4.6 mm× 250 mm,5 μm） column.The mobile phase was composed of methanol and water（with phosphoric acid pH=2） at a flow rate of 1.0 ml/min.The wavelength of UV detector was 360 nm and column temperature was 30℃.Results：The linear ranges of Quercetin,Kaempferol and Isorhamnetin were 0.050-0.400,0.005-0.040,0.005-0.040 μg,respectively.The contents of Total Flavonoid in three batches of samples were 1 Capsule 6.466,6.455,6.838 mg.Conclusion：This method is sensitive,repeatable and suitable to determine the content of Total Flavonoid in Yinxingye Soft Capsules. |
| |
| Keywords: | Yinxingye Soft Capsules Total Flavonoid Quercetin Kaempferol Isorhamnetin Determination |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
