乳腺癌及其边缘组织中Syk的表达及临床意义

目的探讨乳腺癌及其边缘组织中脾酪氨酸激酶(Syk)活性表达及其与手术切缘的安全性关系。方法用SupervionTM二步免疫组化20例乳腺癌标本及其距癌肿边缘1cm、2cm、3cm组织上、下、内、外象限中的Syk表达率,同时与HE染色作对比。结果Syk的阳性表达率3、2、1cm的组织和癌组织中呈现出逐渐递减的趋势(<0.01);相同边缘距离不同象限的Syk阳性表达率无统计学意义(>0.05)。结论Syk表达的趋势性差异提示在乳腺癌周围组织中存在着分子边界,距离肿块3cm可定为乳腺癌的安全切缘。

Expression of sky in breast carcinoma and periphertal tissues and its significance

Objective To study the spleen tyrosine kinase(Syk) activity expression and molecular margin in the tumor center peripheral tissues of patients with breast tumors,and to explore the security of tumor surgical margins. Methods To ect the expressions of Syk in tumor tissues ,the approximal peripheral tissues around the breast quadrants,away 1cm,2cm,3cm approximalperipheral tissues of 20 breast carcinomas with the Supervio-nTM immunohistochemistry,making atoxylin-eosin dyeing (HE) simultaneously. Results The gene ex-pression in breast carcinoma tissues and its surrounding sue at different distance (1,2,3cm) were detected from sur-gical margin of 20 patients with breast cancers. The result of unochemical staining showed that Syk positive ex-pression indicated a tendency of decreasing current from surrounding sue by 3,2,1 cm to tumor tissue( <0.01). Syk positive expression ratio in equal distance of all breast quadrants resented no statistical significanc(e >0.05) Conclusion The differential expression of Syk in breast cancer tissue and surrounding tissue indicate the pre-sence of molecular margin around breast cancer tissue. Considering Syk expressions,3 can be defined as safe mol-ecular margin of lumpectomy.