胃癌组织中T细胞亚群及其ICOS分子的表达及意义

目的探讨胃癌组织中T细胞共刺激分子ICOS及其亚群的表达及意义。方法应用流式细胞术检测38例胃癌和21例健康人（对照组）外周血T细胞亚群及其共刺激分子ICOS的表达。结果胃癌组与对照组比较：CD3^＋T细胞表达（53．61±13．84）％和（72.07±7．83）％，P〈0．01；CD3^＋CD4^＋T细胞表达（29．84±9．71）％和（38．79±5．08）％，P〈0．01；CD3^＋ICOS^＋T细胞表达（25．80±10．56）％和（O．82±0．98）％，P〈0．01；CD3^＋CD8^＋ICOS^＋T细胞表达（1.57±1．99）％和（0．02±0．04）％，P〈0．01；CD3^＋CD8^＋ICOS^-T细胞表达（16．06±6．94）％和（20．56±6．54）％，P〈0．05。胃癌组患者手术前和手术后1周外周血T细胞亚群的差异无统计学意义（P〉0．05）。结论胃癌患者T细胞数量明显减少，T细胞共刺激分子ICOS表达增高，CIM’T细胞显著减少。

Expression of inducible costimulator and variance of T cell subpopulations in patients with gastric cancer

Objective To investigate the role of expression of T cell costimulatory molecule ICOS and variance of T cell subpopulations in the development and prognosis of gastric cancer. Methods The peripheral blood lymphocytes were tested for T cell subpopulations and T cell costimulatory molecule ICOS by flow cytometry in 38 patients with gastric cancer and 21 healthy peoples as control group. Results Compared gastric cancer group with control group：expressions of T cell costimulatory molecule ICOS were（25.80 ±10.56） vs（0. 82 ± 0.98） %, P 〈 0. 01 ; expression percentage of total T cell（ CD3 ^＋ ） were （ 53.61±13.84） vs （72.07 ± 7.83 ） %, P 〈 0.01 ; expression percentage of CD4^ ＋ T ceil （ CD4 ^＋ CD3^ ＋ ） were （ 29.84± 9.71 ） vs （ 38.79 ±5.08 ） %, P 〈 0.01 ; expression percentage of CTL cell （ CD8 ^＋ ICOS ^＋ CD3 ^＋ ） were （ 1.57 ±1. 99） vs （0.02 ± 0.04） %, P 〈 0. 01 ; expression percentage of CD8^ inhibitory T cell （ CD8^ICOS^ - CD3^） ratio were （ 16.6 ± 6.93, ） vs （ 20.56 ± 6.54） % ,P 〈 0.05. No difference of expression was found in peripheral T cell subpopulations of postoperative patients with gastric cancer after one week （ P 〉 0.05 ）. Conclusion T cell number decreased, and T cell eostimulatory molecule ICOS expression incrensed in patients with gastric cancer. CD4 ^＋ T ceil subpopulation is significantly decreased in patients with gastric cancer.